Recombinant Human GFR alpha-like Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9697-GR

R&D Systems, part of Bio-Techne
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9697-GR-050
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Key Product Details

Source

NS0

Accession #

Q6UXV0

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all GFR alpha-like Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human GFR alpha-like protein
Human GFR alpha-like
(Ser19-Glu351)
Accession # Q6UXV0		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser19

Predicted Molecular Mass

64 kDa

SDS-PAGE

62-90 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human GFR alpha‑like Fc Chimera is coated at 0.1 μg/mL, 100 μL/well, it binds to Recombinant GDF-15 (Catalog # 9279-GD) with an ED50 of 0.1-0.7 ng/mL.

Scientific Data Images for Recombinant Human GFR alpha-like Fc Chimera Protein, CF

Recombinant Human GFR alpha-like Fc Chimera Protein Bioactivity

Recombinant Human GFR alpha-like Fc Chimera Protein Bioactivity

When Recombinant Human GFR alpha-like Fc Chimera (Catalog # 9697-GR) is immobilized at 0.1 µg/mL, 100 µL/well, it binds to Recombinant Human GDF-15 (Catalog # 9279-GD) with an ED50 of 0.1-0.7 ng/mL.

Formulation, Preparation and Storage

9697-GR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GFR alpha-like

GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins (1). Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain. Over the extracellular domain, human GFRAL shares 72% and 71%  identity with mouse and rat GFRAL respectively. It is expressed on both fetal and adult hindbrain neurons of the CNS (3), and would appear to function as an anti-apoptotic molecule during neuronal stress. GFRAL is a functional receptor for GDF-15, facilitating weight-loss functions of the protein through c-Ret downstream signaling (2-4). GFRAL and GDF-15 signaling is implicated in diet-based obesity and insulin resistance (2-4).

References

  1. Li, Z. et al. (2005) J. Neurochem. 95:361.
  2. Mullican, S. et al. (2017) Nat. Med 23:1150.
  3. Yang, L. et al. (2017) Nat. Med 23: 1158.
  4. Emmerson, P. et al (2017) Nat. Med 23:1215.

Long Name

Glial Cell line-derived Neurotrophic Factor Receptor alpha-like

Alternate Names

GFR alpha like, GFRAL, GRAL

Entrez Gene IDs

389400 (Human); 404194 (Mouse); 501023 (Rat)

Gene Symbol

GFRAL

UniProt

Q6UXV0

Additional GFR alpha-like Products

Product Documents for Recombinant Human GFR alpha-like Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Human GFR alpha-like Fc Chimera Protein, CF

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