Recombinant Human GLP-1R His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10956-GL

R&D Systems, part of Bio-Techne
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10956-GL-050
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Key Product Details

Source

CHO

Accession #

P43220.2

Conjugate

Unconjugated

Applications

Bioactivity

View all GLP-1R Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human GLP-1R protein
Ala2-Glu139, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala2 & Arg24

Predicted Molecular Mass

17 kDa & 15 kDa

SDS-PAGE

40-50 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When GLP-1 peptide (Tocris, Catalog # 5374) is immobilized at 0.250 µg/mL (100 µL/well), Recombinant Human GLP-1R His-tag binds with an ED50 of 5.00-30.0 ng/mL.

Scientific Data Images for Recombinant Human GLP-1R His-tag Protein, CF

Recombinant Human GLP-1R His-tag Protein Binding Activity.

When GLP-1 peptide (5374) is immobilized at 0.250 µg/mL (100 µL/well), Recombinant Human GLP-1R His-tag Protein (Catalog # 10956-GL) binds with an ED50 of 5.00-30.0 ng/mL.

Recombinant Human GLP-1R His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human GLP-1R His-tag Protein (Catalog # 10956-GL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 40-50 kDa.

Formulation, Preparation and Storage

10956-GL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GLP-1R

Glucagon-like Peptide 1 Receptor (GLP1R) is a member of the Class B or secretin receptor-like family of G protein-coupled receptors (GPCRs) (1-3). Activation of GLP1R signaling regulates secretion of insulin from pancreatic beta -cells in a glucose-dependent manner (4). Human GLP1R consists of an N-terminal, extracellular domain (ECD) containing 6 conserved cysteine residues and a transmembrane domain with seven membrane-spanning alpha-helices, characteristic of GPCRs (1, 3, 5). The mature ECD of human GLP1R shares 87% and 86% amino acid identity with mouse and rat GLP1R, respectively. GLP1R is expressed in pancreas, lung, heart, kidney, hypothalamus, and stomach and it is a receptor for Glucagon-like Peptide, a regulator of insulin secretion by pancreatic beta cells (2, 5, 6). GLP1R agonists, including GLP1, Boc5, and Cpd1, have been targeted for research and used as type 2 diabetes treatments (6, 7). GLP1R is also expressed in the brain where it is involved in the control of appetite (7).

References

  1. Ma, H. et al. (2020) Cell Research. 30:1140.
  2. Song, G. et al. (2017) Nature. 546:312.
  3. Hollenstein, K. et al. (2013) Trends Pharmacol Sci. 35:12.
  4. Drucker, D.J. et al. (1987) PNAS. 84(10):3434.
  5. Donnelly, D. (2012) Br. J. Pharmacol. 166:27.
  6. Simo, R. and Hernandez, C. (2017) Diabetes. 66:1453.
  7. Holst, J.J. (2004) Expert Opinion on Emerging Drugs. 9:155.

Long Name

Glucagon-like Peptide 1 Receptor

Alternate Names

GLP1R

Entrez Gene IDs

2740 (Human); 14652 (Mouse)

Gene Symbol

GLP1R

UniProt

P43220.2

Additional GLP-1R Products

Product Documents for Recombinant Human GLP-1R His-tag Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human GLP-1R His-tag Protein, CF

For research use only

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