Recombinant Human gp130 Fc Chimera Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI10929

Biotinylated
R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
AVI10929-025
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

CHO

Accession #

AAA59155.1

Structure / Form

Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all gp130 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human gp130 protein
Human gp130
(Glu23-Ile618)
Accession # AAA59155.1		 IEGRMD Human IgG1
(Pro100-Lys330)		 Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu23

Predicted Molecular Mass

96 kDa

SDS-PAGE

115-127 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Biotinylated Recombinant Human gp130 Fc Chimera Avi-tag is captured on a Streptavidin Coated Plate (Catalog # CP004), it binds to Recombinant Human IL-6 (Catalog # 7270-IL) in the presence of Recombinant Human IL-6R alpha (Catalog # 227-SR). The ED50 for this binding is 1-10 ng/mL.

Scientific Data Images for Recombinant Human gp130 Fc Chimera Avi-tag Protein, CF

Biotinylated Recombinant Human gp130 Fc Chimera Avi-tag Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human gp130 Fc Chimera Avi-tag Protein (Catalog # AVI10929) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 115-127 kDa and 230-250 kDa, respectively.

Formulation, Preparation and Storage

AVI10929
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: gp130

gp130 (glycoprotein 130, also known as IL-6RB and IL-6ST) is a subunit of receptor complexes for several cytokines that mediate highly diverse biological processes. These include functionally and structurally related cytokines within the IL-6 and IL-12 family including IL-6, IL-11, IL-27, IL-35, IL-39, leukemia inhibitory factor (LIF), oncostatin M (OSM), ciliary neurotrophic factor (CNTF), cardiotrophin-1 (CT-1), novel neutrophin-1/B cell stimulating factor-3 or cardiotrophin-like cytokine (CLC) and neuropoetin (NP) (1, 2). gp130 is ubiquitously expressed in all cell types. Depending on the cytokine, binding of ligand leads to the formation of signalling complexes that trigger a sophisticated signalling machinery downstream of gp130. Binding of IL-6 (IL-11) to either the membrane-anchored or soluble IL-6 R (IL-11 R) initiates the association of IL-6 R (IL-11 R) with gp130 which then undergoes homo-dimerization and signal transduction. With other IL-6 family cytokines, such as LIF and OSM, signal transduction is triggered by the hetero-dimerization of gp130 and LIF R or OSM R (3-6). Mature human CP130 consists of a 597 amino acid (aa) extracellular region (ECD), a 22 aa transmembrane domain, and a 277 aa cytoplasmic region. Within the ECD, human gp130 shares 73% and 75% aa sequence identity with mouse and rat gp130, respectively. Soluble gp130, which apparently arises either from proteolytic cleavage of the membrane-bound receptor or from alternative splicing, has been detected in human serum. At the present time, the in vivo functions of soluble gp130 are not clearly understood. In in vitro experiments, natural or recombinant soluble gp130 has been shown to have inhibitory effects on OSM and CNTF activities. Our Avi-tag Biotinylated Recombinant Human gp130 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Xu, S. and N. Neamati (2013) Expert Opin. Ther. Targets 17:1303.
  2. Ritter, K. et al. (2020) Cells 9:2695.
  3. Martinez-Perez, C, et al. (2021) J. Pers. Med. 11:618.
  4. Silver, J.S. and C.A. Hunter (2010) J. Leukoc. Biol. 88:1145.
  5. Narazaki, M. et al. (1993) Blood 82:1120.
  6. Taga, T. and T. Kishimoto (1997) Annu. Rev. Immunol. 15:797.

Long Name

Glycoprotein 130

Alternate Names

CD130, IL6ST

Entrez Gene IDs

3572 (Human); 16195 (Mouse); 25205 (Rat); 101926138 (Cynomolgus Monkey)

Gene Symbol

IL6ST

UniProt

AAA59155.1

Additional gp130 Products

Product Documents for Recombinant Human gp130 Fc Chimera Avi-tag Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human gp130 Fc Chimera Avi-tag Protein, CF

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.