Recombinant Human Guanylyl Cyclase C Fc Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI10575

Biotinylated
R&D Systems, part of Bio-Techne
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

CHO

Accession #

P25092.2

Structure / Form

Biotinylated via Avi-tag.

Conjugate

Biotin

Applications

Bioactivity

View all Guanylyl Cyclase C/GUCY2C Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Guanylyl Cyclase C/GUCY2C protein
Human Guanylyl Cyclase C/GUCY2C
(Ser24-Gln430)
Accession # P25092.2		 IEGRMD Human IgG1
(Pro100-Lys330)		 Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser24

Predicted Molecular Mass

74 kDa

SDS-PAGE

105-120 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Human Guanylyl Cyclase C Antibody  (Catalog # MAB2157) is immobilized at 100 ng/mL (100 µL/well), Biotinylated Recombinant Human Guanylyl Cyclase C Fc Chimera Avi-tag binds with an ED50 of 0.500-3.00 ng/mL.

Measured by its ability to inhibit neurite outgrowth of E16-E18 rat embryonic cortical neurons.
2.50 μg/mL of protein is able to significantly inhibit neurite outgrowth.

Scientific Data Images for Recombinant Human Guanylyl Cyclase C Fc Avi-tag Protein, CF

Biotinylated Recombinant Human Guanylyl Cyclase C Fc Chimera Avi-tag Binding Activity.

When Human Guanylyl Cyclase C Antibody (MAB2157) is immobilized at 100 ng/mL (100 µL/well), Biotinylated Recombinant Human Guanylyl Cyclase C Fc Chimera Avi-tag (Catalog # AVI10575) binds with an ED50 of 0.500-3.00 ng/mL.

Biotinylated Recombinant Human Guanylyl Cyclase C Fc Chimera Avi-tag Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human Guanylyl Cyclase C Fc Chimera Avi-tag Protein (Catalog # AVI10575) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 105-120 kDa, and 210-240 kDa, respectively.

Formulation, Preparation and Storage

AVI10575
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 1.00 mg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Guanylyl Cyclase C/GUCY2C

Guanylyl Cyclase C (GUCY2C), also known as heat-stable enterotoxin receptor, is a type I transmembrane protein of the guanylate cyclase (GC) family and helps regulate intestinal function (1, 8). There are 7 known members of the GC family (GC-A through GC-G) and they catalyze the conversion of guanosine triphosphate to cyclic guanosine monophosphate (cGMP) and pyrophosphate (1-4). Mature human GUCY2C consists of an extracellular domain (ECD) with a ligand binding domain, a transmembrane segment and a cytoplasmic region possessing a pseudokinase domain and a GC catalytic domain. The ECD of mature human GUCY2C shares 70% and 72% amino acid sequence identity with the ECD of mouse and rat GUCY2C, respectively. GUCY2C is primarily expressed in intestinal epithelial cells from the duodenum to rectum and binds to the endogenous gastrointestinal hormones guanylin and uroguanylin (1-4). Activation of GUCY2C signaling mediates fluid-ion homeostasis, intestinal inflammation, and cell proliferation in a cGMP-dependent manner (5). GUCY2C is also expressed in neurons, where it plays a role in attention deficiency/hyperactive behavior (6). GUCY2C is being investigated as a treatment for several gastrointestinal disorders including irritable bowel syndrome, obesity, colorectal cancer, and chronic idiopathic constipation (7, 8). Our Avi-tag Biotinylated GUCY2C features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Arshad, N. and Visweswariah, S.S. (2012) FEBS Lett. 586:2835.
  2. Potter, L.R. (2011) Cell Signal. 2011 23:1921.
  3. Arshad, N. et al. (2013) J. Biol. Chem. 288:3907.
  4. Gibbons, A.V. et al. (2013) Cancer Res. 73:22.
  5. Lucas, K. et al. (2000) Pharmacol Rev. 52:375.
  6. Gong, R. et al. (2011) Science 333:1642.
  7. Waldman, S.A. and Camilleri, M. (2018) Gut 67:1543.
  8. Kim, G.W. et al. (2013) Trends Endocrinol Metab 24(4):165.

Alternate Names

DIAR6, GC-C, GCC, Guanylate Cyclase C, GUC2C, GUCY2C, MUCIL, STA Receptor, StAR

Entrez Gene IDs

2984 (Human); 14917 (Mouse); 25711 (Rat)

Gene Symbol

GUCY2C

UniProt

P25092.2

Additional Guanylyl Cyclase C/GUCY2C Products

Product Documents for Recombinant Human Guanylyl Cyclase C Fc Avi-tag Protein, CF

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Product Specific Notices for Recombinant Human Guanylyl Cyclase C Fc Avi-tag Protein, CF

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