Recombinant Human HVEM/TNFRSF14 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 356-HV/CF

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R&D Systems, part of Bio-Techne
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356-HV-100/CF
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Key Product Details

Source

NS0

Accession #

Q92956.3

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all HVEM/TNFRSF14 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human HVEM/TNFRSF14 protein
Human HVEM
Pro37-Val202
(Ser108Thr; Ala140Arg)
Accession # Q92956.3		 IEGRMD Human IgG1
(Pro100-Lys330)		 6-His tag
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Pro37

Predicted Molecular Mass

45 kDa (monomer)

SDS-PAGE

60 kDa, reducing conditions

Activity

Measured by its ability to inhibit TNF-beta -mediated cytotoxicity using L-929 mouse fibroblast cells.
The ED50 for this effect is 2.5-10 µg/mL in the presence of 50 pg/mL of Recombinant Human TNF‑ beta/TNFSF1 (Catalog # 211-TB).

Formulation, Preparation and Storage

356-HV/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HVEM/TNFRSF14

HVEM (herpesvirus entry mediator), also known as TNFRSF14 and CD270, is a type I membrane protein in the TNF receptor superfamily, and it can both promote and inhibit T cell activity (1). Mature human HVEM consists of a 164 amino acid (aa) extracellular domain (ECD) with three cysteine-rich domains (CRD), a 21 aa transmembrane segment, and a 60 aa cytoplasmic tail with a TRAF interaction domain (2, 3). Within the ECD, human HVEM shares 55% aa sequence identity with mouse and rat HVEM. Alternative splicing generates an additional isoform with a substitution of the N-terminal 10 amino acids including the signal peptide. HVEM is highly expressed on naïve CD4+ T cells, CD8+ T memory cells, regulatory T cells, dendritic cells, monocytes, and neutrophils (4-8). Its expression declines during effector T cell activation but is up-regulated during Treg activation (4, 5). HVEM functions as a receptor for BTLA, CD160, LIGHT/TNFSF14, and Lymphotoxin-alpha (4, 9-12). Ligation of HVEM by LIGHT triggers T cell, monocyte, and neutrophil activation (8, 10) and contributes to Th1 inflammation and cardiac allograft rejection (13, 14). In contrast, HVEM binding to CD160 or BTLA suppresses T cell and dendritic cell activation (4, 7, 9, 10) and dampens intestinal inflammation (15). HVEM enhances the development of CD8+ T cell memory and Treg function (5, 6). It is additionally expressed on intestinal epithelial cells, where its binding by intraepithelial lymphocyte (IEL) expressed CD160 promotes epitheilal integrity and host defense (16). The herpesvirus envelope glycoprotein gD, which binds HVEM to initiate membrane fusion, can antagonize both BTLA and LIGHT binding (2, 9, 11).

References

  1. del Rio, M.L. et al. (2010) J. Leukoc. Biol. 87:223.
  2. Montgomery, R.I. et al. (1996) Cell 87:427.
  3. Hsu, H. et al. (1997) J. Biol. Chem. 272:13471.
  4. Sedy, J. R. et al. (2005) Nat. Immunol. 6:90.
  5. Tao, R. et al. (2008) J. Immunol. 180:6649.
  6. Steinberg, M.W. et al. (2013) PLoS One 8:e77992.
  7. de Trez, C. et al. (2008) J. Immunol. 180:238.
  8. Heo, S.K. et al. (2006) J. Leukoc. Biol. 79:330.
  9. Gonzalez, L.C. et al. (2005) Proc. Natl. Acad Sci. USA 102:1116.
  10. Cai, G. et al. (2008) Nat. Immunol. 9:176.
  11. Mauri, D.N. et al. (1998) Immunity 8:21.
  12. Harrop, J.A. et al. (1998) J. Biol. Chem. 273:27548.
  13. Wang, J. et al. (2005) J. Immunol. 174:8173.
  14. Ye, Q. et al. (2002) J. Exp. Med. 195:795.
  15. Steinberg, M.W. et al. (2008) J. Exp. Med. 205:1463.
  16. Shui, J.W. et al. (2012) Nature 488:222.

Long Name

Herpesvirus Entry Mediator

Alternate Names

ATAR, CD270, LIGHTR, TNFRSF14

Entrez Gene IDs

8764 (Human); 230979 (Mouse); 102137807 (Cynomolgus Monkey)

Gene Symbol

TNFRSF14

UniProt

Q92956.3

Additional HVEM/TNFRSF14 Products

Product Documents for Recombinant Human HVEM/TNFRSF14 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Human HVEM/TNFRSF14 Fc Chimera Protein, CF

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