Recombinant Human IFN-gamma Biotinylated Protein, CF

R&D Systems, part of Bio-Techne | Catalog # BT285

Biotinylated
R&D Systems, part of Bio-Techne
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BT285-050
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Key Product Details

Source

E. coli

Accession #

CAA31639.1

Structure / Form

Biotinylated via Amines

Conjugate

Biotin

Applications

Bioactivity

View all IFN-gamma Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human IFN-gamma protein
Gln24-Gln166, with a N-terminal Met

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

16.9 kDa

SDS-PAGE

14-20 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human IFN-gamma R1/CD119 (Catalog # 673-IR/CF) is immobilized at 3 µg/mL (100 µL/well), Biotinylated Recombinant Human IFN-gamma (Catalog # BT285) binds with an ED50 of 3.00-27.0 ng/mL.

Scientific Data Images for Recombinant Human IFN-gamma Biotinylated Protein, CF

Biotinylated Recombinant Human IFN‑ gamma Protein Binding Activity.

When Recombinant Human IFN-gamma R1/CD119 (673-IR/CF) is immobilized at 3 µg/mL (100 µL/well), Biotinylated Recombinant Human IFN-gamma Protein (Catalog # BT285) binds with an ED50 of 3.00-27.0 ng/mL.

Biotinylated Recombinant Human IFN‑ gamma Protein SDS-PAGE.

2 μg/lane of Recombinant Human IFN-gamma Biotinylated Protein (Catalog # BT285) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 14-20 kDa.

Formulation, Preparation and Storage

BT285
Formulation Supplied as a 0.2 μm filtered solution in Sodium Succinate, Mannitol and Tween®® 80.
Reconstitution Reconstitute at 0.2 mg/mL in sterile, deionized water.  
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: IFN-gamma

Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature human IFN-gamma exists as a non-covalently linked homodimer of 20-25 kDa variably glycosylated subunits (3). It shares 90% amino acid (aa) sequence identity with rhesus IFN-gamma, 59%‑64% with bovine, canine, equine, feline, and porcine IFN‑ gamma, and 37%‑43% with cotton rat, mouse, and rat IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI ( alpha subunits) which then interact with IFN-gamma RII ( beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, up‑regulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).

References

  1. Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97.
  2. Pestka, S. et al. (2004) Immunol. Rev. 202:8.
  3. Gray, P.W. and D.V. Goeddel (1982) Nature 298:859.
  4. Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. 92:5401.
  5. Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995.
  6. Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163.
  7. McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125.
  8. Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247.
  9. Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.

Long Name

Interferon gamma

Alternate Names

IFG, IFI, IFNG, IFNgamma

Entrez Gene IDs

3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)

Gene Symbol

IFNG

UniProt

CAA31639.1

Additional IFN-gamma Products

Product Documents for Recombinant Human IFN-gamma Biotinylated Protein, CF

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Product Specific Notices for Recombinant Human IFN-gamma Biotinylated Protein, CF

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