Recombinant Human IFN-gamma (HEK293-expressed) Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10067-IF

R&D Systems, part of Bio-Techne
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10067-IF-100
10067-IF-025
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Key Product Details

Source

HEK293

Accession #

NP_000610.2

Structure / Form

Noncovalent homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all IFN-gamma Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human IFN-gamma protein
Met1-Gln166

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln24 inferred from enzymatic pyroglutamate treatment revealing Asp25.

Predicted Molecular Mass

16.8 kDa

SDS-PAGE

19-25 kDa, reducing conditions

Activity

Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129.
The ED50 for this effect is 0.06-0.36 ng/mL.

Scientific Data Images for Recombinant Human IFN-gamma (HEK293-expressed) Protein, CF

Recombinant Human IFN-gamma (HEK293-expressed) Protein Bioactivity

Recombinant Human IFN-gamma (HEK293-expressed) Protein Bioactivity

Recombinant Human IFN-gamma (Catalog # 10067‑IF) demonstrates anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 0.06‑0.36 ng/mL
Recombinant Human IFN-gamma (HEK293-expressed) Protein SDS-PAGE

Recombinant Human IFN-gamma (HEK293-expressed) Protein SDS-PAGE

2 μg/lane of Recombinant Human IFN‑ gamma (HEK293-expressed) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 19-25 kDa.

Formulation, Preparation and Storage

10067-IF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: IFN-gamma

Interferon-gamma (IFN-gamma), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature human IFN-gamma exists as a non-covalently linked homodimer of 20-25 kDa variably glycosylated subunits (3). Glycosylation of IFN-gamma at sites Asn25 and Asn97 is critical for protease resistance (4, 5). The mature protein shares 36% and 35% amino acid (aa) sequence identity with mouse and rat IFN-gamma, respectively. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (6, 7). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (8). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, up-regulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (8, 9). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (10, 11). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (9).

References

  1. Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97.
  2. Pestka, S. et al. (2004) Immunol. Rev. 202:8.
  3. Gray, P.W. and D.V. Goeddel (1982) Nature 298:859.
  4. Sareneva, T. et al. (1994) J. Biochem. 303:831.
  5. Sareneva, T. et al. (1995) J. Biochem. 308:9.
  6. Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. 92:5401.
  7. Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995.
  8. Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163.
  9. McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125.
  10. Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247.
  11. Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.

Long Name

Interferon gamma

Alternate Names

IFG, IFI, IFNG, IFNgamma

Entrez Gene IDs

3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)

Gene Symbol

IFNG

UniProt

NP_000610.2

Additional IFN-gamma Products

Product Documents for Recombinant Human IFN-gamma (HEK293-expressed) Protein, CF

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Product Specific Notices for Recombinant Human IFN-gamma (HEK293-expressed) Protein, CF

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