Recombinant Human IFN-gamma R1/CD119 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11030-IR

R&D Systems, part of Bio-Techne
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11030-IR-100
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Key Product Details

Source

HEK293

Accession #

P15260.1

Conjugate

Unconjugated

Applications

Bioactivity

View all IFN-gamma R1/CD119 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human IFN-gamma R1/CD119 protein
Glu18-Gly245, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu18

Predicted Molecular Mass

27 kDa

SDS-PAGE

36-46 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.

When Recombinant Human IFN-gamma (HEK293-expressed)  (Catalog # 10067-IF) is immobilized at 3 µg/mL (100 µL/well), Recombinant Human IFN-gamma R1/CD119 His-tag (Catalog # 11030-IR) binds with an ED50 of 0.0750-0.900 µg/mL.

Scientific Data Images for Recombinant Human IFN-gamma R1/CD119 His-tag Protein, CF

Recombinant Human IFN-gamma R1/CD119 His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human IFN-gamma R1/CD119 His-tag Protein (Catalog # 11030-IR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 36-46 kDa.

Recombinant Human IFN-gamma R1/CD119 His-tag Protein Binding Activity.

When Recombinant Human IFN-gamma (HEK293-expressed) Protein (10067-IF) is immobilized at 3 µg/mL (100 µL/well), Recombinant Human IFN-gamma R1/CD119 His-tag Protein (Catalog # 11030-IR) binds with an ED50 of 0.0750-0.900 µg/mL.

Formulation, Preparation and Storage

11030-IR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 400 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-gamma R1/CD119

Interferon gamma receptor 1 (IFNGR1), along with IFNGR2, are type II cytokine receptors that combine to form a high affinity signaling complex with the type II interferon, IFNG. Mature human IFNGR1 consists of an extracellular domain (ECD) with 2 Ig-like domains, a transmembrane domain and an intracellular domain with both Jak1 and Stat1 binding motifs. The ECD of human IFNGR1 shares 50% amino acid sequence identity with mouse IFNGR1. The IFNG signaling complex is formed by 2 IFNGR1 subunits binding one IFNG dimer directly, and then 2 IFNGR2 molecules further stabilizing the receptor complex. Complex formation then triggers a signaling cascade that culminates in the transcription of the interferon stimulated genes (ISGs) and additional transcription factors. Ultimately, IFNGR1 mediated signaling regulates several biological processes including innate and acquired immune response, apoptosis and cell cycle progression. IFNGR1 is constitutively expressed in most cell types and deletions or mutations to IFNGR1 result in reduced resistance to bacterial, parasitic, and viral infection.

References

  1. Bhat, M.Y. et al. (2018) J. Cell Commun Signal 12:745.
  2. de Weerd, N.A. and Nguyen, T. (2012) Immunol Cell Biol. 90:483.
  3. Mendoza, J.L. et al. (2019) Nature 567:56.
  4. Blouin, C.M. and Lamaze, C. (2013) Front Immunol. 4:267.
  5. Alspach, E. et al. (2019) Cold Spring Harb Perspect Biol. 11:a028480.

Long Name

Interferon gamma Receptor 1

Alternate Names

CD119, IFN-gR1, IFNgammaR1, IFNGR1

Entrez Gene IDs

3459 (Human); 15979 (Mouse)

Gene Symbol

IFNGR1

UniProt

P15260.1

Additional IFN-gamma R1/CD119 Products

Product Documents for Recombinant Human IFN-gamma R1/CD119 His-tag Protein, CF

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Product Specific Notices for Recombinant Human IFN-gamma R1/CD119 His-tag Protein, CF

For research use only

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