Recombinant Human IGF-I R/IGF1R Fc Chimera Protein, CF

Name: Recombinant Human IGF-I R/IGF1R Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 11655-GR

R&D Systems, part of Bio-Techne | Catalog # 11655-GR

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Product Datasheet / COA / SDS

Key Product Details

Source:	HEK293
Structure / Form:	Disulfide linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human IGF-I R/IGF1R Fc Chimera Protein, CF

Technical Data
Citations / Reviews

Product Specifications for Recombinant Human IGF-I R/IGF1R Fc Chimera Protein, CF

Source

Human embryonic kidney cell, HEK293-derived human IGF-I R/IGF1R protein

Human IGF1R (Glu31-His935) Accession # P08069.1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Glu31 (apha subunit), Asp741 (beta subunit)

Predicted Molecular Mass

130 kDa (single chain), 80 kDa (alpha subunit) and 49 kDa (beta subunit)

SDS-PAGE

130 kDa (single chain), 80 kDa (alpha subunit) and 49 kDa (beta subunit)

Activity

Measured by its binding ability in a functional ELISA. Recombinant Human IGF-I R/IGF1R Fc Chimera (Catalog # 11655-GR binds to Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1) with an ED₅₀ of 3.00-45.0 ng/mL.

Scientific Data Examples for Recombinant Human IGF-I R/IGF1R Fc Chimera Protein, CF

	Recombinant Human IGF-I R/IGF1R Fc Chimera Protein Binding Activity. In a functional ELISA, Recombinant Human IGF-I R/IGF1R Fc Chimera Protein (Catalog # 11655-GR) binds to Recombinant Human IGF-I/IGF-1 (291-G1) with an ED₅₀ of 3.00-45.0 ng/mL.
	Recombinant Human IGF-I R/IGF1R Fc Chimera Protein SDS-PAGE. 2 μg/lane of (Catalog # 11655-GR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 160-180 kDa, 110-125 kDa and 70-80 kDa, under reducing conditions.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11655-GR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 100 μg/mL in water.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IGF-I R/IGF1R

IGF-I receptor is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha and two beta subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The alpha subunit of IGF-I receptor is extracellular while the beta subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. The IGF-I receptor is highly expressed in all cell types and tissues. Essentially all of the biological activities of IGF-I and II have been shown to be mediated via IGF-I R.

Item	Value
References	Rechler, M.M. and S.P. Nissley (1990) in Insulin-Like Growth Factors. Sporn, M.B. and A.B. Roberts (eds): Peptide Growth Factors and Their Receptors I, New York: Springer-Verlag, p. 263.
Long Name	Insulin-like Growth Factor I Receptor
Entrez Gene IDs	3480 (Human)
Alternate Names	CD221, CD221 antigen, EC 2.7.10, EC 2.7.10.1, IGF-1R, IGF-I R, IGF-I receptor, IGF-IR, IGF1R, IGFIR, IGFR, Insulin-like growth factor I receptor, JTK13, MGC142170, MGC142172, MGC18216, insulin-like growth factor 1 receptor, soluble IGF1R variant 1, soluble IGF1R variant 2

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Recombinant Human IGF-I R/IGF1R Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11655-GR

Key Product Details

Key Product Details

Product Specifications for Recombinant Human IGF-I R/IGF1R Fc Chimera Protein, CF

Scientific Data Examples for Recombinant Human IGF-I R/IGF1R Fc Chimera Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: IGF-I R/IGF1R

Customer Reviews for Recombinant Human IGF-I R/IGF1R Fc Chimera Protein, CF

Product Documents for Recombinant Human IGF-I R/IGF1R Fc Chimera Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

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