Recombinant Human IGF-I R/IGF1R (His-tagged) Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 305-GR
Analyzed by SEC-MALS
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human IGF-I R/IGF1R protein
Met1-Asn932 with a C-terminal 10-His tag
Met1-Asn932 with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Glu31 and Asp741
Predicted Molecular Mass
104 kDa (single chain), 81 kDa ( alpha subunit) and 23 kDa ( beta subunit)
SDS-PAGE
150 kDa, 120 kDa and 48 kDa, reducing conditions
Activity
Measured by its binding ability in a functional ELISA.
When Recombinant Human IGF‑I R/IGF1R is present at 0.5 μg/mL, the concentration of Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1) that produces 50% of the optimal binding response is found to be approximately 2‑10 ng/mL.
When Recombinant Human IGF‑I R/IGF1R is present at 0.5 μg/mL, the concentration of Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1) that produces 50% of the optimal binding response is found to be approximately 2‑10 ng/mL.
Scientific Data Images for Recombinant Human IGF-I R/IGF1R (His-tagged) Protein, CF
Recombinant Human IGF-I R/IGF1R (Histidine-tagged) Protein SEC-MALS.
Recombinant Human IGF1R/C-His Protein (Catalog # 305-GR) has a molecular weight (MW) of 296.9 kDa as analyzed by SEC-MALS, suggesting that this protein is a heterotetramer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).Formulation, Preparation and Storage
305-GR
| Formulation | Lyophilized from a 0.2 μm filtered solution in NaH2PO4, NaCl, EDTA and Tween®-20 with Trehalose. |
| Reconstitution |
Reconstitute at 100 μg/mL in sterile PBS.
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| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: IGF-I R/IGF1R
IGF-I receptor is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha and two beta subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The alpha subunit of IGF-I receptor is extracellular while the beta subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. The IGF-I receptor is highly expressed in all cell types and tissues. Essentially all of the biological activities of IGF-I and II have been shown to be mediated via IGF-I R.
References
- Rechler, M.M. and S.P. Nissley (1990) Insulin-Like Growth Factors, in Peptide Growth Factors and Their Receptors I, M.B. Sporn and A.B. Roberts, editors, Springer-Verlag, New York, New York, p. 263.
Long Name
Insulin-like Growth Factor I Receptor
Alternate Names
CD221, IGF-1R, IGF-IR, IGF1R, IGFIR
Entrez Gene IDs
3480 (Human)
Gene Symbol
IGF1R
UniProt
Additional IGF-I R/IGF1R Products
Product Documents for Recombinant Human IGF-I R/IGF1R (His-tagged) Protein, CF
Product Specific Notices for Recombinant Human IGF-I R/IGF1R (His-tagged) Protein, CF
For research use only
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