Recombinant Human IGF-I R/IGF1R Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 391-GR

R&D Systems, part of Bio-Techne
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391-GR-050
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Key Product Details

Source

NS0

Accession #

P08069

Conjugate

Unconjugated

Applications

Binding Activity

View all IGF-I R/IGF1R Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human IGF-I R/IGF1R protein
Met1-Asn932

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu31 and Asp741

Predicted Molecular Mass

102.9 kDa (single chain); 81.0 kDa ( alpha chain) & 21.9 kDa ( beta chain)

SDS-PAGE

160 kDa, 125 kDa, 50 kDa & 40 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Recombinant Human IGF-I R/IGF1R (Catalog # 391-GR) binds to Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1).

Reviewed Applications

Read 1 review rated 5 using 391-GR in the following applications:

Formulation, Preparation and Storage

391-GR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGF-I R/IGF1R

IGF-I receptor is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha and two beta subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The alpha subunit of IGF-I receptor is extracellular while the beta subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. The IGF-I receptor is highly expressed in all cell types and tissues. Essentially all of the biological activities of IGF-I and II have been shown to be mediated via IGF-I R.

References

  1. Rechler, M.M. and S.P. Nissley (1990) in Insulin-Like Growth Factors. Sporn, M.B. and A.B. Roberts (eds): Peptide Growth Factors and Their Receptors I, New York: Springer-Verlag, p. 263.

Long Name

Insulin-like Growth Factor I Receptor

Alternate Names

CD221, IGF-1R, IGF-IR, IGF1R, IGFIR

Entrez Gene IDs

3480 (Human)

Gene Symbol

IGF1R

UniProt

P08069

Additional IGF-I R/IGF1R Products

Product Documents for Recombinant Human IGF-I R/IGF1R Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human IGF-I R/IGF1R Protein, CF

For research use only

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