Recombinant Human IL-18 R beta/IL-1 R7 His-tag Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 11210-AP
Key Product Details
Product Specifications
Source
Human embryonic kidney cell, HEK293-derived human IL-18 R beta/IL-1 R7 protein
Phe20-Arg356, with a C-terminal 6-His tag
Phe20-Arg356, with a C-terminal 6-His tag
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Phe20
Predicted Molecular Mass
39 kDa
SDS-PAGE
46-61 kDa, under reducing conditions.
Activity
Measured by its ability to inhibit IL-18-induced response of KG-1 human acute myelogenous leukemia cells in the presence of soluble Recombinant Human IL-18 R alpha/IL-1 R5 Fc Chimera
(Catalog #
816-LR)
. The ED50 for this effect is 0.300-2.40 µg/mL in the presence of 30.0 µg/mL of soluble Recombinant Human IL-18 R alpha/IL-1 R5 Fc Chimera and 40 ng/mL of Recombinant Human IL-18/IL-1F4 Protein.
Scientific Data Images for Recombinant Human IL-18 R beta/IL-1 R7 His-tag Protein, CF
Recombinant Human IL‑18 R beta/IL‑1 R7 His-tag Protein Bioactivity.
Measured by its ability to inhibit IL-18-induced response of KG-1 human acute myelogenous leukemia cells in the presence of soluble Recombinant Human IL-18 R alpha/IL-1 R5 Fc Chimera (816-LR). The ED50 for this effect is 0.300-2.40 µg/mL in the presence of 30.0 µg/mL of soluble Recombinant Human IL-18 R alpha/IL-1 R5 Fc Chimera and 40 ng/mL of Recombinant Human IL-18/IL-1F4 Protein.
Recombinant Human IL‑18 R beta/IL‑1 R7 His-tag Protein SDS-PAGE.
2 μg/lane of Recombinant Human IL‑18 R beta/IL‑1 R7 His-tag Protein (Catalog # 11210-AP) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 46-61 kDa.Formulation, Preparation and Storage
11210-AP
| Formulation | Supplied as a 0.2 μm filtered solution in PBS with Trehalose. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: IL-18 R beta/IL-1 R7
IL-18, originally described as an interferon-gamma inducing factor (IGIF), is a member of the IL-1 family of cytokines that has multiple immunoregulatory functions. It has potent IFN-gamma inducing activities and plays a key role in the activation of T helper type 1 (Th1) responses. The functional IL-18 receptor complex consists of two components, the IL-18 R alpha (IL-1 R5) and IL-18 R beta (also termed IL-1 R7 and AcPL) subunits. Both subunits are members of the IL-1 receptor superfamily. Although IL-18 R alpha by itself binds IL-18 with low-affinity and IL-18 R beta does not bind IL-18 in vitro, co-expression of IL-18 R alpha and IL-18 R beta is required for high-affinity binding and IL-18 responsiveness. Human IL-18 R beta cDNA encodes a 599 amino acid (aa) residue precursor type I membrane protein with a 14 aa signal peptide, a 342 aa extracellular region containing three immunoglobulin-like domains, a single transmembrane domain and a 222 aa cytoplasmic domain. Human and mouse IL-18 R beta share 65% aa sequence identity. The expression of IL-18 R beta parallels that of IL-18 R alpha and is detected in numerous tissues including lung, spleen, leukocytes and colon.
References
- Born, T.L. et al. (1998) J. Biol. Chem. 273:29445.
- Okamura, H. et al. (2000) in Cytokine Reference, Vol. 2:1605, Academic Press.
Long Name
Interleukin 18 Receptor beta
Alternate Names
AcPL, CD218b, IL-1 R7, IL-18Rb, IL18R beta, IL18RAP
Gene Symbol
IL18RAP
UniProt
Additional IL-18 R beta/IL-1 R7 Products
Product Documents for Recombinant Human IL-18 R beta/IL-1 R7 His-tag Protein, CF
Product Specific Notices for Recombinant Human IL-18 R beta/IL-1 R7 His-tag Protein, CF
For research use only
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