Recombinant Human Jagged 1 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10957-JG

R&D Systems, part of Bio-Techne
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10957-JG-050
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Key Product Details

Source

HEK293

Accession #

P78504.3

Conjugate

Unconjugated

Applications

Bioactivity

View all Jagged 1 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Jagged 1 protein
Ser32-Ser1046, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser32

Predicted Molecular Mass

111 kDa

SDS-PAGE

125-150 kDa, reducing conditions.

Activity

Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. Nobta, M. et al. (2005) J. Biol. Chem. 280:15842.
The ED50 for this effect is 0.750-4.50 μg/mL.

Scientific Data Images for Recombinant Human Jagged 1 His-tag Protein, CF

Recombinant Human Jagged 1 His-tag Protein Bioactivity.

Immobilized Recombinant Human Jagged 1 His-tag (Catalog # 10957-JG) enhances Recombinant Human/Mouse/Rat BMP-2 (355-BM) induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 0.750-4.50 µg/mL.

Recombinant Human Jagged 1 His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human Jagged 1 His-tag Protein (Catalog # 10957-JG) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 125-150 kDa.

Formulation, Preparation and Storage

10957-JG
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Jagged 1

Jagged 1 is a 180 kDa type I transmembrane glycoprotein and member of the Delta-Serrate-Lag-2 (DSL) family of ligands that activate LIN12/Notch proteins. Human Jagged 1 is synthesized as a 1218 amino acid (aa) precursor that contains a 33 aa signal sequence, a 1034 aa extracellular domain (ECD), a 26 aa transmembrane segment, and a 125 aa cytoplasmic region. The ECD contains a DSL domain (aa 185-229), a cysteine-rich region, 15 EGF-like repeats, of which many bind calcium, and nine potential sites for N-linked glycosylation. This product is an N-terminal ECD fragment that includes DSL domain and the first 3 EGF-like domains. Mature human Jagged 1 is 97% and 96% aa identical to mature mouse and rat Jagged 1, respectively. Jagged 1 is widely expressed in adult and fetal tissues. Jagged-Notch signaling specifies cell fate, regulates pattern formation, defines boundaries between different cell types, and modulates cell proliferation and differentiation, especially during hematopoiesis, myogenesis, neurogenesis, and development of vasculature (1-8). Mutations in human Jagged 1 are the cause of Alagille syndrome, an autosomal-dominant disorder characterized by intrahepatic cholestasis and abnormalities of heart, eye, vertebrae, as well as characteristic facial appearance (9, 10).

References

  1. Sainson, R.C. and A.L. Harris (2008) Angiogenesis 11:41.
  2. Cordle, J. et al. (2008) Nat. Struct. Mol. Biol. 15:849.
  3. Artavamis-Tsokanas, S. et al. (1999) Science 284:770.
  4. Lai, E.C. (2004) Development 131:965.
  5. Milner, L.A. et al. (1994) Blood 83:2057.
  6. Nyfeler, Y. et al. (2005) EMBO J. 24:3504.
  7. Linheng, L. et al. (1998) Immunity 8:43.
  8. Iso, T. et al. (2003) Arterioscler. Thromb. Vasc. Biol. 23:543.
  9. Oda, T. et al. (1997) Nat. Genet. 16:235.
  10. Oda, T. et al. (1997) Genomics 43:376.

Alternate Names

CD339, JAG1

Entrez Gene IDs

182 (Human); 16449 (Mouse); 29146 (Rat)

Gene Symbol

JAG1

UniProt

P78504.3

Additional Jagged 1 Products

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