Recombinant Human Lgr6 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8470-GP

R&D Systems, part of Bio-Techne
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8470-GP-050
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Key Product Details

Source

CHO

Accession #

Q9HBX8

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Lgr6 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Lgr6 protein
Human Lgr6
(Ala25-Ala567)
Accession # Q9HBX8		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>80%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala25

Predicted Molecular Mass

87 kDa

SDS-PAGE

90-108 kDa, reducing conditions

Activity

Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells.
The ED50 for this effect is 2.5-12.5 μg/mL in the presence of 10 ng/mL Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).

Scientific Data Images for Recombinant Human Lgr6 Fc Chimera Protein, CF

Lgr6 Enhances Wnt Signaling.

Lgr6 Enhances Wnt Signaling.

Recombinant Human Lgr6 induces Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 2.5-12.5 µg/mL in the presence of 10 ng/mL Recombinant Mouse Wnt-3a (Catalog # 1324-WN).

Formulation, Preparation and Storage

8470-GP
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lgr6

Lgr6 (leucine‑rich repeat G protein‑coupled receptor 6) is an approximately 100 kDa 7‑transmembrane glycoprotein in the Lgr family of cell surface receptors. Lgrs has been identified as a marker for specific types of stem cells in tissues that are subject to constant self-renewal such as skin, gastrointestinal tract, and lungs (1, 2). The 544 amino acid (aa) N-terminal extracellular domain (ECD) of mature human Lgr6 contains 15 LRRs flanked by N-terminal and C-and C-terminal LRR domains. The ECD is followed by a 7-TM region and a 137 aa C-terminal cytoplasmic domain (3). Within the N-terminal ECD, human Lgr6 shares 92% and 93% aa sequence identity with mouse and rat Lgr6, respectively. Alternative splicing generates an additional isoform with a substitution for the LRR N-terminal domain and a deletion of LRR3-6. Lgr6 is expressed on hair follicle stem cells that give rise to sebaceous glands, interfollicular epidermis, and hair follicles (4-6). It is also expressed on alveolar stem cells that differentiate into lung epithelium (7). It is often up-regulated in human gastric cancers (8). Lgr6 binds to R‑Spondins 1-4 and can enhance Wnt/beta-Catenin signaling through phosphorylation of the Wnt co-receptor LRP6 (9, 10).

References

  1. de Lau, W. et al. (2014) Genes Dev. 28:305.
  2. Leushacke, M. and N. Barker (2012) Oncogene 31:3009.
  3. Hsu, S.Y. et al. (2000) Mol. Endocrinol. 14:1257.
  4. Snippert, H.J. et al. (2010) Science 327:1385.
  5. Liao, X.-H. and H. Nguyen (2014) Exp. Dermatol. 23:195.
  6. Nath, M. et al. (2011) Cell Tissue Res. 344:435.
  7. Oeztuerk-Winder, F. et al. (2012) EMBO J. 31:3431.
  8. Steffen, J.S. et al. (2012) Virchows Arch. 461:355.
  9. Gong, X. et al. (2012) PLoS ONE 7:e37137.
  10. de Lau, W. et al. (2011) Nature 476:293.

Long Name

Leucine-rich Repeat Containing G Protein-coupled Receptor 6

Alternate Names

FLJ14471, gonadotropin receptor, GPCR, leucine-rich repeat containing G protein-coupled receptor 6, leucine-rich repeat-containing G protein-coupled receptor 6, leucine-rich repeat-containing G-protein coupled receptor 6, VTS20631

Entrez Gene IDs

59352 (Human); 329252 (Mouse); 498233 (Rat)

Gene Symbol

LGR6

UniProt

Q9HBX8

Additional Lgr6 Products

Product Documents for Recombinant Human Lgr6 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Human Lgr6 Fc Chimera Protein, CF

For research use only

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