Recombinant Human METTL11A Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7159-MT

R&D Systems, part of Bio-Techne
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7159-MT-020
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Key Product Details

Source

E. coli

Accession #

Q9BV86

Conjugate

Unconjugated

Applications

Enzyme Activity

View all alpha-N-terminal Methyltransferase 1A/METTL11A Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human alpha-N-terminal Methyltransferase 1A/METTL11A protein
Thr2-Arg223, with an N-terminal Met and 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

26 kDa

SDS-PAGE

25-26 kDa, reducing conditions

Activity

Measured by its ability to methylate the synthetic peptide Pro-Pro-Lys-Gln-Gln-Leu-Ser-Lys-Tyr.
The specific activity is >2.5 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

7159-MT
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: alpha-N-terminal Methyltransferase 1A/METTL11A

METTL11A, also named N‑terminal RCC1 methyltransferase (NRMT), is an alpha‑N‑methyltransferase that methylates the N‑terminal amino group of target proteins containing the N‑terminal motif [Ala/Pro/Ser]-Pro-Lys when the initiator Met is cleaved (1). It is responsible for N‑terminal methylation of RCC1, KLHL31, MYL2, MYL3, RB1, RPL23A and SET. NRMT lacks a SET domain but possesses a Rossman‑like a/b fold. The residues Asn169, Asp178, Asp181, and Ser183 of NRMT are important for substrate binding (2). RCC1 (Ran guanine nucleotide‑exchange factor) is the first protein for which any biological function of alpha‑N‑methylation by NRMT has been identified (3, 4). The multi‑spindle phenotype associated with either NRMT knockdown or methylation‑defective RCC1 mutants demonstrated the importance of alpha‑N‑methylation of RCC1 for normal bipolar spindle formation and chromosome aggregation (2). NRMT is robustly overexpressed in gastrointestinal cancers.

References

  1. Webb, K. J. et al. (2010) Biochemistry 49:5225.
  2. Christine, S. E. et al. (2010) Nature 466:1125.
  3. Chen, T. et al. (2007) Nature Cell Biol. 9:596.
  4. Hao, Y. et al. (2008) J. Cell. Biol. 182:827.

Long Name

alpha-N-terminal Protein Methyltransferase 1A

Alternate Names

AD-003, C9orf32, NRMT, NTM1A, NTMT1

Entrez Gene IDs

28989 (Human); 66617 (Mouse); 362103 (Rat)

Gene Symbol

NTMT1

UniProt

Q9BV86

Additional alpha-N-terminal Methyltransferase 1A/METTL11A Products

Product Documents for Recombinant Human METTL11A Protein, CF

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Product Specific Notices for Recombinant Human METTL11A Protein, CF

For research use only

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