Recombinant Human mGluR8 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9534-UR

R&D Systems, part of Bio-Techne
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9534-UR-100
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Key Product Details

Source

CHO

Accession #

O00222-1

Structure / Form

Disulfide linked homodimer

Conjugate

Unconjugated

View all mGluR8 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human mGluR8 protein
Gln34-Ser514, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained. Gln34 inferred from enzymatic pyroglutamate treatment revealing Glu35

Predicted Molecular Mass

55 kDa

SDS-PAGE

54-70 kDa, reducing conditions

Activity

Bioassay data are not available.

Formulation, Preparation and Storage

9534-UR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: mGluR8

Metabotropic glutamate receptors (mGluRs) are members of the G-protein-coupled receptor (GPCR) superfamily, modulating glutamate neurotransmission in the central and peripheral nervous systems through GTP-binding proteins (1). Structurally, members of this family are characterized by a large N-terminal extracellular domain (ECD), seven transmembrane domains, and a cytoplasmic C-terminal domain that is variable in length. Two ECDs dimerize together and large conformational changes are induced when agonists bind to one or both domains (2). The C-terminal region is subject to alternative splicing, regulation by phosphorylation, and interacts directly with a G-protein to modulate protein-protein interactions (2, 3). The receptors are subdivided into three groups (I–III) based on sequence homology, signal transduction and pharmacological properties (1, 2). The Group III receptors include mGLuRs 4, 6, 7 and 8 (2). Mature human mGluR8 is 875 amino acids in length, including a 550 amino acid (aa) N-terminal ECD (4). Within N-terminal ECD, human mGluR8 shares 98.4% and 98.5% aa sequence identity with mouse and rat mGluR8, respectively.

References

  1. Conn P.J. and Pin J.P. (1997) Annu. Rev. Pharmacol. Toxicol. 37:205.
  2. Niswender C.M. and Conn P.J. (2010) Annu. Rev. Pharmacol. Toxicol. 50: 295.
  3. Pin J.P. and Duvoisin R. (1995) Neuropharmacology. 34:1.
  4. Wu S. et al. (1998) Brain Res. Mol. Brain Res. 53:88.

Long Name

Metabotropic Glutamate Receptor 8

Alternate Names

GPRC1H, GRM8

Entrez Gene IDs

2918 (Human); 14823 (Mouse); 60590 (Rat)

Gene Symbol

GRM8

UniProt

O00222-1

Additional mGluR8 Products

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Product Specific Notices for Recombinant Human mGluR8 Protein, CF

For research use only

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