Recombinant Human MMP-9 Protein, CF Best Seller

R&D Systems, part of Bio-Techne | Catalog # 911-MP

R&D Systems, part of Bio-Techne
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911-MP-010
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Key Product Details

Source

CHO

Accession #

P14780

Structure / Form

Pro form

Conjugate

Unconjugated

Applications

Enzyme Activity

View all MMP-9 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human MMP-9 protein
Ala20-Asp707 (Gln279Arg)

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.1 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala20

Predicted Molecular Mass

77 kDa

SDS-PAGE

93 kDa, reducing conditions

Activity

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2 (Catalog # ES001).
The specific activity is >1,300 pmol/min/µg, as measured under the described conditions.

Reviewed Applications

Read 8 reviews rated 4.6 using 911-MP in the following applications:

Scientific Data Images for Recombinant Human MMP-9 Protein, CF

Recombinant Human MMP-9 Protein Enzyme Activity

Recombinant Human MMP-9 Protein Enzyme Activity

Recombinant Human MMP-9 (Catalog # 911-MP) is measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2 (Catalog # ES001).

Formulation, Preparation and Storage

911-MP
Formulation Supplied as a 0.2 μm filtered solution in Tris, CaCl2, NaCl and Brij-35.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: MMP-9

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-9 (gelatinase B) can degrade a broad range of substrates including gelatin, collagen types IV and V, elastin and proteoglycan core protein. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP-9 is produced by keratinocytes, monocytes, macrophages and PMN leukocytes. MMP-9 is present in most cases of inflammatory responses. Structurally, MMP-9 maybe be divided into five distinct domains: a pro-domain which is cleaved upon activation, a gelatin-binding domain consisting of three contiguous fibronectin type II units, a catalytic domain containing the zinc binding site, a proline-rich linker region, and a carboxyl terminal hemopexin-like domain. In addition to the human enzyme, the recombinant mouse MMP-9 is also available (Catalog # http://www.rndsystems.com/product_results.aspx?k=909-MM">909-MM).

Long Name

Matrix Metalloproteinase 9

Alternate Names

CLG4B, Gelatinase B, GELB, MANDP2, MMP9

Entrez Gene IDs

4318 (Human); 17395 (Mouse); 81687 (Rat); 102117693 (Cynomolgus Monkey)

Gene Symbol

MMP9

UniProt

P14780

Additional MMP-9 Products

Product Documents for Recombinant Human MMP-9 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human MMP-9 Protein, CF

For research use only

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