Recombinant Human Nectin-2/CD112 Fc Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI9317

Biotinylated
R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
AVI9317-050
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

HEK293

Accession #

NP_002847.1

Structure / Form

Disulfide-linked homodimer

Conjugate

Biotin

Applications

Bioactivity

View all Nectin-2/CD112 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Nectin-2/CD112 protein
Human Nectin-2/CD112
(Gln32-Leu360)
Accession # NP_002847.1		 IEGRMD Human IgG1
(Pro100-Lys330)		 Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE with silver staining, under reducing conditions.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln32 inferred from enzymatic pyroglutamate treatment revealing Asp33.

Predicted Molecular Mass

62 kDa

SDS-PAGE

74-84 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human DNAM-1 Fc Chimera  (Catalog # 666-DN) is immobilized at 1.00 µg/mL (100 µL/well), Biotinylated Recombinant Human Nectin-2/CD112 Fc Chimera Avi-tag binds with an ED50 of 8.00-48.0 ng/mL.

Scientific Data Images for Recombinant Human Nectin-2/CD112 Fc Avi-tag Protein, CF

Biotinylated Recombinant Human Nectin‑2/CD112 Fc Chimera Avi-tag Protein Binding Activity.

When Recombinant Human DNAM-1 Fc Chimera (666-DN) is immobilized at 1.00 µg/mL (100 µL/well), Biotinylated Recombinant Human Nectin-2/CD112 Fc Chimera Avi-tag (Catalog # AVI9317) binds with an ED50 of 8.00 - 48.0 ng/mL.

Biotinylated Recombinant Human Nectin‑2/CD112 Fc Chimera Avi-tag Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human Nectin‑2/CD112 Fc Chimera Avi-tag Protein (Catalog # AVI9317) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 74 - 84 kDa and 148 - 168 kDa, respectively.

Formulation, Preparation and Storage

AVI9317
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nectin-2/CD112

Nectin-2, also known as Poliovirus receptor-related 2 (PRR2), is a member of the Nectin family which are Ca++-independent immunoglobulin (Ig)-like cell adhesion molecules (CAMs) that organize intercellular junctions (1). The Nectin family is comprised of 4 family members and 5 nectin‑like molecules and they are structurally homologous to the poliovirus receptors (2). Mature human Nectin‑2 consists of an extracellular domain (ECD) with three immunoglobulin-like domains, a single transmembrane segment, and a cytoplasmic domain bind the F-actin–binding protein afadin (3). Within the ECD, human Nectin‑2 shares 72% amino acid (aa) sequence identity with mouse Nectin‑2. Alter native splicing generates an isoform with a truncated cytoplasmic tail (1). Nectin-2 localizes to adherens junctions between neurons, endothelial cells, epithelial cells, and fibroblasts (3, 4). It forms homodimers in cis, followed by dimers in trans (between cells) (4). It does not cis‑dimerize with other Nectins but forms cis‑dimers between its two splice forms. Notably, a Nectin-2 cis‑dimer on one cell can heterodimerize with a Nectin‑3 cis‑dimer on a neighboring cell (4). Nectin‑2 additionally binds to DNAM-1/CD226 on NK cells and triggers NK cell cytolytic activity (5, 6). Nectin‑2 is known to bind pseudorabies virus and herpes simplex virus-2 (HSV-2), but not HSV-1 or poliovirus (4, 7). Nectin‑2 is a component of cardiac intercalated discs and limits fibrosis and dysfunction resulting from pressure overload (8). Our Avi-tag Biotinylated Nectin‑2 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Eberle, F. et al. (1995) Gene 159:267.
  2. Samanta, D. and S.C. Almo (2015) Cell. Mol. Life Sci. 72:645.
  3. Samanta, D. et al. (2012) PNAS 109:14836.
  4. Struyf, F. et al. (2002) J. Virol. 76:12940.
  5. Bottino, C. et al. (2003) J. Exp. Med. 198:557.
  6. Pende, D. et al. (2005) Mol. Immunol. 42:463.
  7. Warner, M.S. et al. (1998) Virology 246:179.
  8. Satomi-Kobayashi, S. et al. (2009) Hypertension 54:825.

Long Name

Poliovirus Receptor Related 2

Alternate Names

CD112, HVEB, MPH, Nectin2, PRR2, PVRL2

Entrez Gene IDs

5819 (Human); 19294 (Mouse); 102137456 (Cynomolgus Monkey)

Gene Symbol

NECTIN2

UniProt

NP_002847.1 (Human)

Additional Nectin-2/CD112 Products

Product Documents for Recombinant Human Nectin-2/CD112 Fc Avi-tag Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human Nectin-2/CD112 Fc Avi-tag Protein, CF

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.