Recombinant Human NF-L Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9715-NF

R&D Systems, part of Bio-Techne
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9715-NF-050
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Key Product Details

Source

E. coli

Accession #

P07196

Conjugate

Unconjugated

View all NF-L Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human NF-L protein
Ser2-Asp338, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser2

Predicted Molecular Mass

39 kDa

SDS-PAGE

41 kDa, reducing conditions

Activity

Bioassay data are not available.

Formulation, Preparation and Storage

9715-NF
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution
Reconstitute at 500 μg/mL in 4 mM HCl..

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NF-L

Neurofilament light polypeptide (NF-L), also known as 68 kDa neurofilament protein, and neurofilament triplet L protein, is an approximately 68 kDa member of the neuronal intermediate filament family that plays a role in the maintenance of neuronal caliber and possibly the pathogenesis of motor neuron diseases (1, 2). Out of the three intermediate filament proteins (NF-L, NF-M, NF-H), NF-L is the most abundant. NF-L is a 542 amino acid (aa) cytoskeletal protein which consists of a 91 aa N-terminal head, a 304 aa alpha-helical rod, and a 147 aa C-terminal tail (3). Within the extracellular domains, human NF-L shares 95% and 96% sequence identity with mouse and rat NF-L, respectively.

References

  1. Monteiro, MJ. et al. (1990) J. Cell. Biol. 111:1543.
  2. Xu, Z. et al. (1993) Cell. 73:23.
  3. Adebola, A. et al. (2015) Hum. Mol. Genet. 24:2163.

Long Name

Neurofilament Protein, Light Chain

Alternate Names

CMT2E, NEFL, NF68, NFL

Entrez Gene IDs

4747 (Human)

Gene Symbol

NEFL

UniProt

P07196

Additional NF-L Products

Product Documents for Recombinant Human NF-L Protein, CF

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human NF-L Protein, CF

For research use only

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