Recombinant Human NKp46/NCR1 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10517-NK

R&D Systems, part of Bio-Techne
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10517-NK-050
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Key Product Details

Source

CHO

Accession #

NP_004820.2

Conjugate

Unconjugated

Applications

Bioactivity

View all NKp46/NCR1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human NKp46/NCR1 protein
Gln22-Gln254, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained. Gln22 inferred from enzymatic pyroglutamate treatment revealing Gln23

Predicted Molecular Mass

27 kDa

SDS-PAGE

31-45 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human NKp46/NCR1 His-tag (Catalog # 10517-NK) is immobilized at 2.0 μg/mL, 100 μL/well, the concentration of Recombinant Human Vimentin (Catalog # 2105-VI) that produces 50% of the optimal binding response is approximately 50-500 ng/mL.

Scientific Data Images for Recombinant Human NKp46/NCR1 His-tag Protein, CF

Recombinant Human NKp46/NCR1 His-tag Protein Binding Activity.

When Recombinant Human NKp46/NCR1 His-tag (Catalog # 10517-NK) is immobilized at 2.0 μg/mL, 100 μL/well, the concentration of Recombinant Human Vimentin (2105-VI) that produces 50% of the optimal binding response is approximately 50-500 ng/mL.

Recombinant Human NKp46/NCR1 His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human NKp46/NCR1 His-tag Protein (Catalog # 10517-NK) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 31-45 kDa.

Formulation, Preparation and Storage

10517-NK
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NKp46/NCR1

NKp46, also called NCR1, along with NKp30 and NKp44, are activating receptors that are collectively known as the natural cytotoxicity receptor (NCR) family (1). NKp46 is a type I transmembrane glycoprotein with two extracellular Ig-like domains followed by a short stalk region in the extracellular domain (ECD), a transmembrane domain containing a positively charged amino acid residue, and a short cytoplasmic tail. The mature ECD of human NKp46 shares 61% and 63% amino acid sequence identity with mouse and rat NKp46, respectively. NKp46 is unique among the NCRs in its evolutionary conservation between species (2). NCRs are expressed almost exclusively by NK cells and play a major role in triggering some of the key lytic activities of NK cells. Through its positive charge in the transmembrane domain, NKp46 associates with the ITAM-bearing signal adapter proteins, CD3 zeta and Fc epsilon R1 gamma (3, 4). NKp46 is involved with tumor cell recognition and has the capacity to bind to viral hemagglutinins, such as that of influenza and Sendai viruses (5, 6). NKp46 is also crucial for the development of type 1 diabetes, the clearance of Streptococcus pneumoniae, killing stellate cells in attenuated liver fibrosis, and the regulation of graft-versus-host disease and allergic response (7-10).

References

  1. Hadad, U. et al. (2015) Front Immunol. 6:495.
  2. Biassoni, R. et al. (1999) Eur J Immunol. 29:1014.
  3. Augugliaro, R. et al. (2003) Eur. J. Immunol. 33:1235.
  4. Moretta, A. et al. (2001) Annu. Rev. Immunol. 19:197.
  5. Halfteck, G. et al. (2009) J. Immunol. 182:2221.
  6. Mendelson, M. et al. (2010) J. Virol. 84:3789.
  7. Gur, C. et al. (2011). J. Immunol. 187:3096.
  8. Elhaik-Goldman, S. et al. (2011). PLoS One. 6:e23472.
  9. Gur, C. et al. (2012) Gut. 61:885.
  10. Ghadially, H. et al. (2013) Eur. J. Immunol. 43:3006.

Alternate Names

CD335, Ly94, MAR-1, NCR1

Entrez Gene IDs

9437 (Human); 17086 (Mouse); 100141419 (Porcine); 102115479 (Cynomolgus Monkey)

Gene Symbol

NCR1

UniProt

NP_004820.2

Additional NKp46/NCR1 Products

Product Documents for Recombinant Human NKp46/NCR1 His-tag Protein, CF

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Product Specific Notices for Recombinant Human NKp46/NCR1 His-tag Protein, CF

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