Recombinant Human PSG1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9334-P1

R&D Systems, part of Bio-Techne
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9334-P1-050
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Key Product Details

Source

NS0

Accession #

P11464

Conjugate

Unconjugated

Applications

Bioactivity

View all PSG1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human PSG1 protein
Gln35-Pro419, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln35 & Val36

Predicted Molecular Mass

44 kDa

SDS-PAGE

64-77 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human SLITRK5 (Catalog # 2587-SK) is coated at 500 ng/mL, Recombinant Human PSG1 (Catalog # 9334-P1) binds with an ED50 = 50-300 ng/mL.

Scientific Data Images for Recombinant Human PSG1 Protein, CF

Recombinant Human PSG1 Protein Binding Activity

Recombinant Human PSG1 Protein Binding Activity

When Recombinant Human SLITRK5 (Catalog # 2587-SK) is coated at 500 ng/mL, Recombinant Human PSG1 (Catalog # 9334-P1) binds with an ED50 = 50-300 ng/mL.

Formulation, Preparation and Storage

9334-P1
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with trehalose.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 2 weeks, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PSG1

Pregnancy-specific beta-1-glycoprotein 1 (PSG1), also called SP1, PSG1 or B1G1 and designated CD66f, is a secreted glycoprotein of the human PSG family within the CEA (carcinoembryonic antigen) superfamily (1-3). PSG1 shares 84-91% amino acid (aa) sequence identity with the most similar family members, PSG3, 4, 6, 7, and 8. Mature PSG1 contains a V-type Ig-like domain that is important for adhesion (N1), and three C2-type Ig-like domains (A1, A2 and B2). Three potential isoforms vary at the extreme C-terminus. Although rodents express pregnancy-specific glycoproteins, identity with human PSG proteins is low and limited to the N1 domain (3). PSG1 is one of the most abundant fetal proteins found in maternal blood during pregnancy. It is secreted by syncytiotrophoblasts and exerts proangiogenic effect by inducing VEGF-A secretion and formation of tubes in endothelial cells (4). PSG1 also serves an immunomodulatory function through
up-regulation of TGF-beta in macrophages, monocytes, and trophoblasts (5). In addition PSG1 has been shown to induce secretion of anti-inflammatory cytokines
IL-10 and IL-6 in human monocytes (6). PSG1 binds Integrin alphaIIb beta3 and inhibits platelet-fibrinogen interactions (7). Recent studies also suggest PSG1's involvement in cancer biology and correlation with chemotherapy resistance in breast cancer (8).

References

  1. Watanabe, S. and J.Y. Chou (1988) J. Biol. Chem. 263:2049.
  2. Leslie, K.K. et al. (1990) Proc. Natl. Acad. Sci. USA 87:5822.
  3. McLellan, A.S. et al. (2005) BMC Evol. Biol. 5:39.
  4. Lisboa, F. et al. (2011) J Biol Chem. 286:7577.
  5. Ha, C. et al. (2010) Biol Rep. 83:27.
  6. Snyder, S. et al. (2001) Am J Reprod Immunol. 45:205.
  7. Shanley D. et al. (2013) PLoS One. 8:e57491.
  8. He, D. et al. (2016) Clin Sci (Lond). 130:2267.

Long Name

Pregnancy-specific beta-1-glycoprotein 1

Alternate Names

B1G1, CD66f, DHFRP2, PBG1, PS-beta-G-1, PSbG1, PSG-1, PSG95, PSGGA

Entrez Gene IDs

5669 (Human)

Gene Symbol

PSG1

UniProt

P11464

Additional PSG1 Products

Product Documents for Recombinant Human PSG1 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human PSG1 Protein, CF

For research use only

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