Recombinant Human SCG3 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10374-SC

R&D Systems, part of Bio-Techne
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10374-SC-050
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Key Product Details

Source

CHO

Accession #

Q8WXD2.3

Conjugate

Unconjugated

Applications

Bioactivity

View all SCG3 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human SCG3 protein
Phe20-Leu468, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe20

Predicted Molecular Mass

52 kDa

SDS-PAGE

50-70 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human SCG3 His-tag (Catalog # 10374-SC) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human Chromogranin A His-tag  (Catalog # 10422-CH) binds with an ED50 of 50-400 ng/mL.

Scientific Data Images for Recombinant Human SCG3 His-tag Protein, CF

Recombinant Human SCG3 His-tag Protein Binding Activity

Recombinant Human SCG3 His-tag Protein Binding Activity

When Recombinant Human SCG3 His-tag (10374-SC) is immobilized at 1 μg/mL (100 μL/well), Recombinant Human Chromogranin A His-tag (10422-CH) binds with an ED50 of 50-400 ng/mL.
Recombinant Human SCG3 His-tag Protein SDS-PAGE

Recombinant Human SCG3 His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human SCG3 His-tag (Catalog # 10374-SC) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 50-70 kDa.

Formulation, Preparation and Storage

10374-SC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SCG3

Secretogranin III, also known as SCG3, is a member of the chromogranin-secretogranin family of neuroendocrine secretory proteins, collectively referred to as granins (1). Additional members of this family include secretogranin II, IV, and V, chromogranin A and B, and a few additional related proteins (1, 2). SCG3 is synthesized as an N-glycosylated protein and two isoforms can be generated from alternative splicing (2). Mature SCG3 contains three functional regions: an N-terminal cholesterol-binding domain, a chromogranin A binding domain and a C-terminal carboxypeptidase E binding domain (3). Human SCG3 shares 88% and 87% amino acid sequence identity with mouse and rat SCG3, respectively. Granins are highly acidic proteins that are a major component of secretory granules of endocrine, neuroendocrine, and neuronal cells (4-6). Calcium binding at low pH and intermolecular aggregation plays a role in granins' sorting of secretory proteins at the trans-Golgi network (3-6). Within the trans-Golgi network, SCG3 binds to chromogranin A (CgA), membrane-associated carboxypeptidase E (CPE), and cholesterol-rich membrane domain (3-5). SCG3 has been implicated in diabetic retinopathy (DR) and retinopathy of prematurity (ROP) as a disease-associated endothelial ligand, thus suggesting SCG3 is a promising target for novel antiangiogenic therapy of DR and ROP (7, 8).

References

  1. Peinado, J. R. et al. (2006) Endocrinology. 147:1408.
  2. Bartolomucci, A. et al. (2011) Endocr. Rev. 32:755.
  3. Hosaka M. et al. (2010) Endocr. J. 57:275.
  4. Sun, M. et al. (2013) Traffic. 14:205.
  5. Hosaka, M. et al. (2004) J. Biol. Chem. 279:3627.
  6. Hosaka, M. et al. (2005) J. Cell Sci. 118:4785.
  7. LeBlanc, M. E. et al. (2017) J. Exp. Med. 214:1029.
  8. Tang, F. et al. (2017) Biochem. Biophys. Res. Commun. 495:781.

Long Name

Secretogranin III

Alternate Names

Secretogranin III, Secretogranin-3, SGIII

Entrez Gene IDs

29106 (Human); 20255 (Mouse)

Gene Symbol

SCG3

UniProt

Q8WXD2.3

Additional SCG3 Products

Product Documents for Recombinant Human SCG3 His-tag Protein, CF

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Product Specific Notices for Recombinant Human SCG3 His-tag Protein, CF

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