Recombinant Human Serpin A3 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 1295-PI
Key Product Details
Product Specifications
Source
Asn26-Ala423, with a C-terminal 10-His tag
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
SDS-PAGE
Activity
The IC50 value is <8 nM, as measured under the described conditions.
Formulation, Preparation and Storage
1295-PI
Formulation | Lyophilized from a 0.2 μm filtered solution in HEPES and NaCl. |
Reconstitution | Reconstitute at 100 μg/mL in sterile 25 mM HEPES and 150 mM NaCl, pH 7.8. |
Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Serpin A3/alpha 1-Antichymotrypsin
Serpin A3 is a member of the Serpin superfamily of the serine protease inhibitors (1). It is synthesized primarily in the liver and secreted as one of the most abundant serpins in plasma (2). It is known to inhibit several serine proteases including chymotrypsin, cathepsin G, chymase, kallikrein 3/prostate specific antigen, and unidentified ectoenzymes that process pro-macrophage stimulating protein (1‑5). Serpin A3 is a major constituent of the plaques associated with Alzheimer’s disease and an inhibitor of amyloid beta peptide degradation (1‑6). Deficiency in Serpin A3 activity due to a point mutation (Leu55Pro) is associated with chronic obstructive pulmonary disease (7). Human Serpin A3 is synthesized as a 423 amino acid precursor (8, 9). The mature protein is secreted and has two forms that differ in their N‑termini (10). One of the forms starting at Asn26 was expressed and purified.
References
- Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
- Kalsheker, N. et al. (2002) Biochem. Soc. Trans. 30:93.
- Duranto, J. et al. (1998) Biochemistry 37:11239.
- Hsieh, M.-C. and B.S. Cooperman (2002) Biochemistry 41:2990.
- Skeel, A. and E.J. Leonard (2001) J. Biol. Chem. 276:21932.
- Abraham, C.R. et al. (2000) Ann. N. Y. Acad. Sci. 920:245.
- Gooptu, B. et al. (2000) Proc. Natl. Acad. Sci. USA 97:67.
- Chandra, T. et al. (1983) Biochemistry 22:5055.
- Morii, M. and J. Travis (1983) J. Biol. Chem. 258:12749.
- Lindmark, B. et al. (1989) Biochim. Biophys. Acta 997:90.
Alternate Names
Entrez Gene IDs
Gene Symbol
UniProt
Additional Serpin A3/alpha 1-Antichymotrypsin Products
Product Documents for Recombinant Human Serpin A3 Protein, CF
Product Specific Notices for Recombinant Human Serpin A3 Protein, CF
For research use only