Recombinant Human Siglec-2/CD22 Fc Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI1968

Biotinylated
R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
AVI1968-050
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

CHO

Accession #

CAA42006.1

Structure / Form

Disulfide-linked homodimer, biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all Siglec-2/CD22 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Siglec-2/CD22 protein
Human Siglec-2/CD22
(Asp20-Arg687)
Accession # CAA42006.1		 IEGRMD Human IgG1
(Pro100-Lys330)		 Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp20

Predicted Molecular Mass

104 kDa

SDS-PAGE

120-140 kDa, under reducing conditions.

Activity

Measured by the ability of the immobilized protein to support the adhesion of human red blood cells. Kelm, S. et al. (1994) Current Biology 4:965.
The ED50 for this effect is 0.1-0.9 μg/mL.

Measured by its binding ability in a functional ELISA.
When Human Siglec-2/CD22 (Epratuzumab) Antibody is immobilized at 0.2 µg/mL (100 µL/well), Biotinylated Recombinant Human Siglec‑2/CD22 Fc Chimera Avi-tag (Catalog # AVI1968) binds with an ED50 of 1.5-18 ng/mL.

Scientific Data Images for Recombinant Human Siglec-2/CD22 Fc Avi-tag Protein, CF

Biotinylated Recombinant Human Siglec‑2/CD22 Fc Chimera Avi-tag Protein Binding Activity.

In a functional ELISA, Biotinylated Recombinant Human Siglec-2/CD22 Fc Chimera Avi-tag Protein (Catalog # AVI1968) binds to Human Siglec-2/CD22 Antibody (MAB19681) with an ED50 of 1.50-18.0 ng/mL.
Recombinant Human Siglec-2/CD22 Fc Avi-tag Protein SDS-PAGE

Recombinant Human Siglec-2/CD22 Fc Avi-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human Siglec-2/CD22 Fc Avi-tag Protein (AVI1968) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 120-140 kDa and 240-280 kDa, respectively.

Formulation, Preparation and Storage

AVI1968
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Siglec-2/CD22

Sialic acid-binding immunoglobulin-like lectin 2 (Siglec-2), also known as B-cell receptor CD22 or B-lymphocyte cell adhesion molecule (BL-CAM), is a I-type (Ig-type) lectin belonging to the sialoadhesin subclass of the immunoglobulin superfamily (1). Fourteen human and nine mouse Siglecs have been characterized and are divided into 2 families: CD33 related and evolutionarily conserved (2, 3). The extracellular domain (ECD) of Siglecs are characterized by an N-terminal Ig-like V-type domain, which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains (1-3). The predominant form of human Siglec-2 contains a N-terminal Ig-like V-type domain, six Ig-like C2-type domains, a transmembrane region and a cytoplasmic tail with six tyrosine residues and four immunoreceptor tyrosine-based inhibition motifs (ITIMs) (1-3). A variant form of Siglec-2 missing two Ig-like C2-type domains along with a truncated cytoplasmic tail has also been identified (4). The mature ECD of human Siglec-2 shares 59% and 58% amino acid sequence identity with mouse and rat Siglec-2, respectively. Siglec-2 is an adhesion molecule that preferentially binds alpha 2,6- linked sialic acid on the same (cis) or adjacent (trans) cells (5). Besides its role as an adhesion molecule, Siglec-2 is a coreceptor that physically interacts with B-cell receptor (BCR), negatively regulating BCR signals by recruiting tyrosine phosphatase SHP-1 to its ITIMs. Phosphorylated Siglec-2 can also interact with other intracellular effector proteins such as Syk, PLC gamma, PI3 kinase and Grb-2, suggesting it may play a role in positive signaling (2). Another function of Siglec-2 is that it mediates the anti-phagocytic effect of alpha2,6-linked sialic acid, and inhibition of Siglec-2 promotes the clearance of myelin debris, amyloid-beta oligomers and alpha-synuclein fibrils in vivo (6). Siglec-2 also plays a role in autoimmunity and has great potential for Siglec-2-based immunotherapeutics for the treatment of autoimmune diseases such as systemic lupus erythematosus (SLE) (7). Our Avi-tag Biotinylated Recombinant Human Siglec-2 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Sato, S. et al. (1996) Immunity. 5:551.
  2. Crocker, P.R. and A. Varki (2001) Trends Immunol. 22:337.
  3. Macauley, M.S. et al. (2014) Nature Rev Imm. 14:653.
  4. Stamenkovic, I. and B. Seed (1990) Nature 345:74.
  5. Collins, B.E. et al. (2004) Proc. Natl. Acad. Sci. 101:6104.
  6. Pluvinage, JV. et al. (2019) Nature. 568:7751.
  7. Clark, E.A. et al. (2018) Front Immunol. 9:2235.

Long Name

Sialic Acid Binding Ig-like Lectin 2

Alternate Names

BL-CAM, CD22, Siglec2

Entrez Gene IDs

933 (Human); 12483 (Mouse); 308501 (Rat); 102130968 (Cynomolgus Monkey)

Gene Symbol

CD22

UniProt

CAA42006.1

Additional Siglec-2/CD22 Products

Product Documents for Recombinant Human Siglec-2/CD22 Fc Avi-tag Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human Siglec-2/CD22 Fc Avi-tag Protein, CF

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.