Recombinant Human SMAC/Diablo Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 789-SM
Key Product Details
Product Specifications
Source
E. coli-derived human SMAC/Diablo protein
Ala56-Asp239, with a C-terminal 6-His tag
Ala56-Asp239, with a C-terminal 6-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
N-terminal Sequence Analysis
Ala56
Predicted Molecular Mass
22 kDa
SDS-PAGE
23 kDa, reducing conditions
Activity
Measured by its ability to reverse the inhibition of DEVD-AFC cleavage activity in cell extracts activated by addition of cytochrome c and dATP.
The IC50 for reversal of XIAP-BIR3 (50 nM) inhibition of DEVD-AFC cleavage in activated cell extracts is <2,000 nM.
Optimal dilutions should be determined by each laboratory for each application.
The IC50 for reversal of XIAP-BIR3 (50 nM) inhibition of DEVD-AFC cleavage in activated cell extracts is <2,000 nM.
Optimal dilutions should be determined by each laboratory for each application.
Formulation, Preparation and Storage
789-SM
| Formulation | Supplied as a 0.2 μm filtered solution in HEPES and NaCl. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: SMAC/Diablo
SMAC (second mitochondria derived activator of caspase)/Diablo promotes caspase activation by interacting with the inhibitor of apoptosis (IAP) proteins in the cytochrome c/Apaf-1/caspase-9 pathway.
SUPPLEMENTARY METHODS
SMAC/Diablo can reverse rhXIAP inhibition of DEVD-AFC cleavage in activated cell lystes.
Recombinant Human XIAP Full Length (Catalog # http://www.rndsystems.com/product_results.aspx?k=822-XF">822-XF) can be used in the Activity Assay Protocol in place of rhXIAP (BIR3). The IC50 for reversal of XIAP (500 nM) inhibition of DEVD-AFC cleavage in activated cell extracts is typically 500-1500 nM.
References
- Du, C. et al. (2000) Cell 102:33.
- Chai, J. et al. (2000) Nature 406:855.
- Srinivasula, S. et al. (2000) J. Biol. Chem. 275:36152.
- Ekert, P. et al. (2001) J. Cell Biol. 152:483.
- Verhagen, A. (2000) Cell 102:43.
Long Name
Second Mitochondria-derived Activator of Caspase
Alternate Names
Diablo
Entrez Gene IDs
56616 (Human)
Gene Symbol
DIABLO
UniProt
Additional SMAC/Diablo Products
Product Documents for Recombinant Human SMAC/Diablo Protein, CF
Product Specific Notices for Recombinant Human SMAC/Diablo Protein, CF
For research use only
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