Recombinant Human SSc5D His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10535-SS

R&D Systems, part of Bio-Techne
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10535-SS-050
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Key Product Details

Source

HEK293

Accession #

NP_001138422.1

Conjugate

Unconjugated

Applications

Bioactivity

View all SSc5D Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human SSc5D protein
Val17-Thr875, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val17

Predicted Molecular Mass

92 kDa

SDS-PAGE

135-153 kDa, under reducing conditions

Activity

Measured by its ability to bind fluorescein-conjugated E. coli Bioparticles.
The ED50 for this effect is 0.2-1 μg/mL.

Scientific Data Images for Recombinant Human SSc5D His-tag Protein, CF

Recombinant Human SSc5D His-tag Protein Binding Activity

Recombinant Human SSc5D His-tag Protein Binding Activity

Recombinant Human SSc5D His-tag (Catalog # 10535-SS) binds fluorescein-conjugated E. coli bioparticles. The ED50 for this effect is 0.2-1 μg/mL.
Recombinant Human SSc5D His-tag Protein SDS-PAGE

Recombinant Human SSc5D His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human SSc5D His-tag Protein (10535-SS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands 120-155 at kDa.

Formulation, Preparation and Storage

10535-SS
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SSc5D

SSc5D (Soluble Scavenger with 5 Domains) is a member of scavenger receptor cysteine-rich (SRCR) superfamily that comprises a group of proteins with one or more domains sharing structural homology with the membrane distal extracellular domain of the macrophage type I scavenger receptor (1). Mature human SSc5D consists of 1573 amino acids (aa), including five SRCR domains at the N-terminus and a heavily glycosylated, mucin-like domain at the C-terminus. Within N‑terminal SRCR domains, human SSc5D shares 95% and 96% aa sequence identity with mouse and rat SSc5D, respectively (1). SSc5D is produced by monocyte/macrophages, T cells and several epithelial cells from placenta, spleen, and colon (1). Many members of the SRCR super family bind to and clear bacteria, fungi or viruses from infected host (2). SSc5D physically interacts with whole bacteria cells especially to E. coli strains. These findings can help fight bacterial infections by discriminating between different bacteria strains and species (3).

References

  1. Goncalves, C.M. et al. (2009) Mol. Immunol. 46:2585.
  2. Martinez, V.G. et al. (2011) Pharmacol. Rev. 63:967.
  3. Bessa Pereira, C. et al. (2016) Front. Immunol. 7:416.

Long Name

Scavenger Receptor Cysteine Rich Family Member With 5 Domains

Alternate Names

S5D-SRCRB

Entrez Gene IDs

284297 (Human)

Gene Symbol

SSC5D

UniProt

NP_001138422.1

Additional SSc5D Products

Product Documents for Recombinant Human SSc5D His-tag Protein, CF

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Certificate of Analysis

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Product Specific Notices for Recombinant Human SSc5D His-tag Protein, CF

For research use only

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