Recombinant Human TEM8/ANTXR1 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 3886-AR

R&D Systems, part of Bio-Techne
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3886-AR-050
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Key Product Details

Source

HEK293

Accession #

Q9H6X2

Structure / Form

Monomer

Conjugate

Unconjugated

Applications

Bioactivity

View all TEM8/ANTXR1 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human TEM8/ANTXR1 protein
Glu33-Ser321, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained. Gln28 inferred from enzymatic pyroglutamate treatment revealing Gly29

Predicted Molecular Mass

34 kDa

SDS-PAGE

38-46 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Anthrax Protective Antigen is coated at 1.5 μg/mL (100 μL/well), the concentration of Recombinant Human TEM8/ANTXR1 that produces 50% optimal binding response is 0.6-3.6 μg/mL.

Scientific Data Images for Recombinant Human TEM8/ANTXR1 His-tag Protein, CF

Recombinant Human TEM8/ANTXR1 His-tag Protein Binding Activity

Recombinant Human TEM8/ANTXR1 His-tag Protein Binding Activity

When anthrax protective antigen (PA) is coated at 1.5 μg/mL, 100 μL/well, Recombinant Human TEM8/ANTXR1 (Catalog # 3886-AR) binds with an ED50 of 0.6-3.6 μg/mL.
Recombinant Human TEM8/ANTXR1 His-tag Protein SDS-PAGE

Recombinant Human TEM8/ANTXR1 His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human TEM8/ANTXR1 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 38 - 46 kDa and 36 - 43 kDa, respectively.

Formulation, Preparation and Storage

3886-AR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: TEM8/ANTXR1

Anthrax toxin receptor 1 (ANTXR1), also known as Tumor endothelial marker 8 (TEM8), is a glycoprotein of the Anthrax Toxin Receptor family that is expressed by endothelial cells. Anthrax toxin receptor 1 contains a 289 amino acid (aa) extracellular domain, a 21 aa transmembrane domain, and a 222 aa cytoplasmic domain. Type I transmembrane isoforms of 564 aa (80‑85 kDa) and 368 aa (60 kDa) and potentially secreted isoforms of 330 aa and 297 aa (45 kDa) are differentially expressed. All diverge at the C-terminal end but share the N-terminal extracellular domain (1). The extracellular domain shares structural similarity with von Willebrand factor type (vWFA) domains, which are characterized by their interactions with ECM components (2, 3). The extracellular domain is involved in reorganization of cell actin cytoskeleton (2, 3). Anthrax Receptor 1 binds Anthrax Protective Antigen with lesser affinity that Anthrax Receptor 2 and induces toxin internalization (4). Anthrax toxin receptor 1 has been implicated in tumor angiogenesis, as its expression has been shown to up-regulate in tumor blood vessels and is characterized as a tumor endothelial marker (5). ANTXR-1 was reported to be an amplifier of Wnt signaling in tumor microenvironment (6). Additionally, Anthrax toxin receptor 1 serves as the receptor for Seneca Valley virus, an oncolytic picornavirus affecting neuroendocrine cancers (7). Human ANTXR1 shares 99% aa identity with mouse and rat and 92% identity with dog and chick ANTXR1 within the extracellular domain.

References

  1. Bradley, Kenneth A, et al. (2001) Nature 414:225.
  2. Hotchkiss, K. et al. (2004). Experimental Cell Research. 305:133.
  3. Whittaker, C. and Hynes, R. (2002). Mol Biol Cell. 13:3369.
  4. Sheng, Fu. et al. (2010) PLOS One. 5(6): e11203.
  5. Carson-Walter, EB. et al. (2001). Cancer Res. 18:6649.
  6. Verma, K. et al. (2011) PLOS One. 6(8):e22334.
  7. Miles, L. et al. (2017). J Clin Invest. 8:2957.

Long Name

Tumor Endothelial Marker 8/Anthrax Toxin Receptor 1

Alternate Names

ANTXR1

Entrez Gene IDs

84168 (Human); 69538 (Mouse); 362393 (Rat)

Gene Symbol

ANTXR1

UniProt

Q9H6X2

Additional TEM8/ANTXR1 Products

Product Documents for Recombinant Human TEM8/ANTXR1 His-tag Protein, CF

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Product Specific Notices for Recombinant Human TEM8/ANTXR1 His-tag Protein, CF

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