Recombinant Human TIM-1/KIM-1/HAVCR Fc Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI9319

Biotinylated
R&D Systems, part of Bio-Techne
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AVI9319-050
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

HEK293

Accession #

AAC39862.1

Structure / Form

Disulfide-linked homodimer, biotinylated via Avi-tag

Conjugate

Biotin

Applications

Binding Activity, Bioactivity

View all TIM-1/KIM-1/HAVCR Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human TIM-1/KIM-1/HAVCR protein
Human TIM-1/KIM-1/HAVCR
(Ser21-Thr288)
Accession # AAC39862.1		 IEGRMD Human IgG1
(Pro100-Lys330)		 Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser21

Predicted Molecular Mass

57 kDa

SDS-PAGE

115-135 kDa, under reducing conditions

Activity

The biotin to protein ratio is greater than 0.7 as determined by the HABA assay.

Measured by its binding ability in a functional ELISA.
When Human TIM-1/KIM-1/HAVCR Affinity Purified Polyclonal Antibody (Catalog # AF1750) is immobilized at 0.2 μg/mL, 100 μl/well, Recombinant Human TIM-1/KIM-1/HAVCR Fc Chimera Avi-tag Protein (Catalog # AVI9319) binds with an ED50 of 1.5-9 ng/mL.

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells.
The ED50 for this effect is 0.12-1.2 μg/mL.

Scientific Data Images for Recombinant Human TIM-1/KIM-1/HAVCR Fc Avi-tag Protein, CF

Recombinant Human TIM-1/KIM-1/HAVCR Fc Avi-tag Protein Binding Activity

Recombinant Human TIM-1/KIM-1/HAVCR Fc Avi-tag Protein Binding Activity

When Human TIM-1/KIM-1/HAVCR Affinity Purified Polyclonal Antibody (AF1750) is immobilized at 0.2 μg/mL, 100 μl/well, Recombinant Human TIM-1/KIM-1/HAVCR Fc Avi-tag Protein (AVI9319) binds with an ED50 of 1.5-9 ng/mL.
Recombinant Human TIM-1/KIM-1/HAVCR Fc Avi-tag Protein SDS-PAGE

Recombinant Human TIM-1/KIM-1/HAVCR Fc Avi-tag Protein SDS-PAGE

2 μg/lane of Biotinylated Recombinant Human TIM-1/KIM-1/HAVCR Fc Chimera Avi-tag (Catalog # AVI9319) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 115-135 kDa and 230-270 kDa, respectively.

Formulation, Preparation and Storage

AVI9319
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIM-1/KIM-1/HAVCR

T cell immunoglobulin and mucin domain 1 (TIM-1), also known as KIM-1 and HAVcr1, is a member of the TIM family which is involved in the regulation of innate and adaptive immune responses (1). TIM-1 is a type I transmembrane protein that contains an N-terminal immunoglobulin-like domain, a mucin domain with O- and N-linked carbohydrates, a transmembrane segment, and a cytoplasmic signaling domain (2). Multiple TIM-1 variants can be produced due to polymorphisms or alternative splicing resulting in deletions in the mucin domain. Within the extracellular domain, human TIM-1 shares 41% amino acid sequence identity with mouse and rat TIM-1. TIM-1 is expressed on splenic B cells, IL-10+ regulatory B cells, CD4+ T cells, mast cells, invariant NKT (iNKT) cells, dendritic cells, kidney epithelium and a broad range of mucosal epithelium (1, 3-5). It is upregulated on activated Th2 cells, after dendritic cell maturation, and on kidney tubular epithelial cells after injury (6-9). Metalloproteinase-mediated cleavage of TIM-1 at the membrane-proximal region results in the release of a soluble form of TIM-1 which is detectable in the urine and in circulation (10). TIM-1 serves as a receptor for phosphatidylserine, LMIR5/CD300b, TIM-1 (homophilic), TIM-4, IgA, and the glycoproteins of a number of enveloped viruses (2, 11-16). Its interaction with phosphatidylserine enables TIM-1 to mediate the phagocytosis of apoptotic cells (12, 13) and iNKT cell activation (17). TIM-1 binding induces the activation of LMIR5-expressing myeloid cells, contributing to tissue homeostasis as well as damage following kidney injury (14). TIM-1 ligation co-stimulates T cell activation and enhances Th2 cytokine production (7, 15). In humans, TIM-1 serves as a cellular entry receptor for various viruses, including hepatitis A virus, Ebolavirus and Marburgvirus (2, 11). Our Avi-tag Biotinylated human TIM-1 Fc Chimera features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Du, P. et al. (2016) J. Immunol. Res. 2016:8605134.
  2. Feigelstock, D. et al. (1998) J. Virol. 72:6621.
  3. Ding, Q. et al. (2011) J. Clin. Invest. 121:3645.
  4. Ma, J. et al. (2011) Biochem. Biophys. Res. Commun. 406:223.
  5. de Souza, A.J. et al. (2005) Proc. Natl. Acad. Sci. USA 102:17113.
  6. Kuehn, E.W. et al. (2002) Am. J. Physiol. Renal Physiol. 283:F1326.
  7. Umetsu, S.E. et al. (2005) Nat. Immunol. 6:447.
  8. Xiao, S. et al. (2011) Eur. J. Immunol. 41:1539.
  9. Ichimura, T. et al. (1998) J. Biol. Chem. 273:4135.
  10. Bailly, V. et al. (2002) J. Biol. Chem. 277:39739.
  11. Kondratowicz, A.S. et al. (2011) Proc. Natl. Acad. Sci. USA 108:8426.
  12. Miyanishi, M. et al. (2007) Nature 450:435.
  13. Kobayashi, N. et al. (2007) Immunity 27:927.
  14. Yamanishi, Y. et al. (2010) J. Exp. Med. 207:1501.
  15. Meyers, J.H. et al. (2005) Nat. Immunol. 6:455.
  16. Tami, C. et al. (2007) J. Virol. 81:3437.
  17. Lee, H.H. et al. (2010) J. Immunol. 185:5225.

Long Name

T Cell Immunoglobulin Mucin-1

Alternate Names

CD365, HAVCR1, KIM-1, TIM1

Entrez Gene IDs

26762 (Human); 171283 (Mouse); 286934 (Rat); 102141332 (Cynomolgus Monkey)

Gene Symbol

HAVCR1

UniProt

AAC39862.1

Additional TIM-1/KIM-1/HAVCR Products

Product Documents for Recombinant Human TIM-1/KIM-1/HAVCR Fc Avi-tag Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human TIM-1/KIM-1/HAVCR Fc Avi-tag Protein, CF

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