Recombinant Human TIM-1/KIM-1/HAVCR His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11157-TM

R&D Systems, part of Bio-Techne
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11157-TM-050
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Key Product Details

Source

HEK293

Accession #

Q96D42.3

Conjugate

Unconjugated

Applications

Bioactivity

View all TIM-1/KIM-1/HAVCR Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human TIM-1/KIM-1/HAVCR protein
Ser21-Gly295, with C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser21

Predicted Molecular Mass

30 kDa

SDS-PAGE

105-125 kDa, under reducing conditions.

Activity

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells.
The ED50 for this effect is 0.320-4.80 µg/mL.

Scientific Data Images for Recombinant Human TIM-1/KIM-1/HAVCR His-tag Protein, CF

Recombinant Human TIM‑1/KIM‑1/HAVCR His-tag Protein Bioactivity.

Recombinant Human TIM-1/KIM-1/HAVCR His-tag Protein (Catalog # 11157-TM) inhibits anti-CD3-induced proliferation of stimulated human T cells. The ED50 for this effect is 0.320-4.80 μg/mL.

Recombinant Human TIM‑1/KIM‑1/HAVCR His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human TIM‑1/KIM‑1/HAVCR His-tag Protein (Catalog # 11157-TM) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 105-125 kDa.

Formulation, Preparation and Storage

11157-TM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIM-1/KIM-1/HAVCR

T cell-immunoglobulin and mucin domain 1 (TIM-1), also known as KIM1 and HAVcr1, type I transmembrane glycoprotein in the TIM subfamily of immunoglobulin (Ig) superfamily molecules. In human, the TIM family is comprised of 3 family members and is involved in the regulation of Th1 and Th2‑cell‑mediated immune responses (1). Mature TIM-1 consists of an extracellular domain (ECD) with one V‑type Ig‑like domain and a mucin domain with O- and N-linked carbohydrates, a transmembrane segment, and a cytoplasmic signaling domain. Within the ECD, human TIM-1 shares 39% amino acid sequence identity with mouse TIM-1. Multiple TIM-1 variants can be produced either through polymorphisms or alternative splicing primarily resulting in deletions in the mucin domain (2). A soluble form of TIM-1, detectable in the urine and in circulation, can be generated through ectodomain cleavage mediated by MMP3 (3, 4). TIM-1 is expressed on splenic B cells, IL-10+ regulatory B cells, CD4+ T cells, mast cells, dendritic cells, kidney epithelium and a broad range of mucosal epithelium (5-7). It is upregulated on activated Th2 cells, after dendritic cell maturation, and on kidney tubular epithelial cells after injury (8-10). TIM-1 ligation induces T cell proliferation and promotes cytokine production (5, 11). TIM-1 serves as a receptor for phosphatidylserine, and its interaction with LMIR5 enables TIM-1 to mediate the phagocytosis of apoptotic cells (12). TIM-1 also serves as a cellular entry receptor for various viruses, including hepatitis A virus, Ebolavirus, Marburgvirus and has been indicated as a possible receptor for SARS-CoV-2 (13-15).

References

  1. Du, P. et al. (2016) J. Immunol. Res. 2016:8605134.
  2. Freeman, G.J. et al. (2010) Immunol. Rev. 235:172.
  3. Lim, A.I. et al. (2012) Int. J. Biochem. Cell Biol. 44:1040.
  4. Bailly, V. et al. (2002) J. Biol. Chem. 277:39739.
  5. Ding, Q. et al. (2011) J. Clin. Invest. 121:3645.
  6. Ma, J. et al. (2011) Biochem. Biophys. Res. Commun. 406:223.
  7. de Souza, A.J. et al. (2005) Proc. Natl. Acad. Sci. USA 102:17113.
  8. Kuehn, E.W. et al. (2002) Am. J. Physiol. Renal Physiol. 283:F1326.
  9. Umetsu, S.E. et al. (2005) Nat. Immunol. 6:447.
  10. Xiao, S. et al. (2011) Eur. J. Immunol. 41:1539.
  11. Meyers, J.H. et al. (2005) Nat. Immunol. 6:455.
  12. Kobayashi, N. et al. (2007) Immunity 27:927.
  13. Tami, C. et al. (2007) J. Virol. 81:3437.
  14. Kondratowicz, A.S. et al. (2011) Proc. Natl. Acad. Sci. USA 108:8426.
  15. Ichimura, T. et al. (2020) medRxiv https://doi.org/10.1101/2020.09.16.20190694.

Long Name

T Cell Immunoglobulin Mucin-1

Alternate Names

CD365, HAVCR1, KIM-1, TIM1

Entrez Gene IDs

26762 (Human); 171283 (Mouse); 286934 (Rat); 102141332 (Cynomolgus Monkey)

Gene Symbol

HAVCR1

UniProt

Q96D42.3

Additional TIM-1/KIM-1/HAVCR Products

Product Documents for Recombinant Human TIM-1/KIM-1/HAVCR His-tag Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human TIM-1/KIM-1/HAVCR His-tag Protein, CF

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