Recombinant Human TREM-1 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10337-TR

R&D Systems, part of Bio-Techne
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10337-TR-050
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Key Product Details

Source

CHO

Accession #

Q9NP99.1

Conjugate

Unconjugated

Applications

Bioactivity

View all TREM-1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human TREM-1 protein
Ala21-Arg200, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala21

Predicted Molecular Mass

21 kDa

SDS-PAGE

30-45 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human PGLYRP1/PGRP-S Protein (Catalog # 2590-PGB) is coated at 2 μg/mL (100 μL/well), the concentration of Recombinant Human TREM-1 His-tag (Catalog # 10337-TR) that produces 50% of the optimal binding response is found to be approximately 50-300 ng/mL.

Scientific Data Images for Recombinant Human TREM-1 His-tag Protein, CF

Recombinant Human TREM-1 His-tag Protein Binding Activity

Recombinant Human TREM-1 His-tag Protein Binding Activity

When Recombinant Human PGLYRP1/PGRP-S Protein (Catalog # 2590-PGB) is coated at 2 µg/mL (100 µL/well), the concentration of Recombinant Human TREM-1 His-tag (Catalog # 10337-TR) that produces 50% of the optimal binding response is found to be approximately 50-300 ng/mL.
Recombinant Human TREM-1 His-tag Protein SDS-PAGE

Recombinant Human TREM-1 His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human TREM-1 His-tag (Catalog # 10337-TR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 30-45 kDa.

Formulation, Preparation and Storage

10337-TR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TREM-1

TREM-1 (Triggering Receptor Expressed on Myeloid cells), also known as CD354, along with TREM-2, and rodent specific TREM-3, comprise a group of Ig superfamily proteins regulating the activation and differentiation of myeloid cells. Human TREM-1 is type I transmembrane protein containing a single Ig-like domain in the extracellular domain (ECD), a transmembrane region and a short cytoplasmic tail. The mature ECD of human TREM-1 shares 45% identity with mouse TREM-1. Several other TREM family members have been reported that are structurally similar but share less than 30% amino acid identity. TREM-1, expressed on monocytes and neutrophils, associates with the adapter protein, DAP12, to deliver an activating signal to elicit and amplify the innate inflammatory response triggered by bacteria (1, 2). A few potential TREM-1 ligands have been identified, including HMGB1 and PGLYRP1 (1-2). HMGB1 (high mobility group Box 1), a ubiquitous nuclear protein, is secreted by myeloid cells during inflammation and has been suggested as an additional TREM-1 ligand (1). However, HMGB1 alone has been found unable to trigger TREM-1 activation and may require co-activating molecules (1). PGLYRP1 (peptidoglycan recognition receptor 1), mainly found in granulocytes, binds to peptidoglycan and cell wall components such as LPS, and has been identified as another potential TREM-1 ligand (1, 2). PGLYRP1, when complexed with PGN, is able to activate TREM-1 and enhance cytokine production in human neutrophils and macrophages (2).

References

  1. Tammaro, et al. (2017) Pharmacology & Therapeutics. 177:81.
  2. Read, et al. (2015) J Immunol. 194:1417.

Long Name

Triggering Receptor Expressed on Myeloid Cells 1

Alternate Names

CD354, TREM1

Entrez Gene IDs

54210 (Human); 58217 (Mouse)

Gene Symbol

TREM1

UniProt

Q9NP99.1

Additional TREM-1 Products

Product Documents for Recombinant Human TREM-1 His-tag Protein, CF

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Product Specific Notices for Recombinant Human TREM-1 His-tag Protein, CF

For research use only

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