Recombinant Human UNC5B Protein Fc Chimera, CF

R&D Systems, part of Bio-Techne | Catalog # 8869-UN

R&D Systems, part of Bio-Techne
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8869-UN-050
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Key Product Details

Source

CHO

Accession #

Q8IZJ1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all UNC5H2/UNC5B Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human UNC5H2/UNC5B protein
Human UNC5H2/UNC5B
(Gly27-Tyr377)
Accession # Q8IZJ1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly27

Predicted Molecular Mass

66 kDa (monomer)

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human UNC5H2/UNC5B Fc Chimera is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Mouse Netrin-1 (Catalog # 1109-N1) that produces 50% optimal binding response is approximately 7-42 ng/mL.

Formulation, Preparation and Storage

8869-UN
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 400 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: UNC5H2/UNC5B

Netrin Receptor UNC5B is one of four related receptors that comprise the UNC5 family and serve as receptors for the axon guidance molecule Netrin-1. UNC5B is a type I transmembrane protein that contains two Ig-like and two thrombospondin domains in its extracellular domain (ECD), and a ZU5 domain, a DCC-binding domain, and a death domain in its intracellular region (1, 2). It is widely expressed in various embryonic and adult tissues including neural, hematopoietic, and immune tissues (1, 3, 4). Human UNC5B has a predicted molecular weight of approximately 104 kDa. Its ECD shares approximately 92% amino acid sequence identity within shared regions in the mouse and rat orthologs, which are termed UNC5H2. UNC5B regulates axon guidance by facilitating Netrin-1-mediated axon repulsion of neuronal growth cones (1). It complexes with the attractive Netrin receptor DCC and switches its response to Netrin-1 from attraction to repulsion (5). UNC5B can also induce growth cone collapse via its associations with the RGM-A receptor Neogenin and the soluble ECD of FLRT3 (2, 6). Outside of the nervous system, UNC5B facilitates Netrin-1-mediated inhibition of capillary branching and leukocyte migration, as well as Netrin-1-mediated promotion of osteoclast differentiation (7-10). UNC5B also functions as a dependence receptor. In the absence of Netrin-1, UNC5B mediates p53-dependent apoptosis via activation of DAPK (11, 12). UNC5B is believed to play a role in tumorgenesis. It has been shown to inhibit tumor cell growth and invasion, and its expression appears to be down-regulated in multiple cancers including colorectal, breast, ovary, uterus, stomach, lung, and kidney (13, 14). Additionally, UNC5B is thought to play a critical role in the development of atherosclerosis and the survival of renal tubular epithelial cells during acute kidney injury (15, 16).

References

  1. Leonardo, E.D. et al. (1997) Nature 386:833.
  2. Hata, K. et al. (2009) J. Cell Biol. 184:737.
  3. Engelkamp, D. (2002) Mech. Dev. 118:191.
  4. Manitt, C. et al. (2004) J. Neurosci. Res. 77:690.
  5. Hong, K. et al. (1999) Cell 97:927.
  6. Yamagishi, S. et al. (2011) EMBO J. 30:2920.
  7. Lu, X. et al. (2004) Nature 432:179.
  8. Ly, N.P. et al. (2005) Proc. Natl. Acad. Sci. USA 102:14729.
  9. Larrivée, B. et al. (2007) Genes Dev. 21:2433.
  10. Mediero, A. et al. (2015) J. Bone Miner. Res. 30:837.
  11. Tanikawa, C. et al. (2003) Nat. Cell Biol. 5:216.
  12. Llambi, F. et al. (2005) EMBO J. 24:1192.
  13. Thiébault, K. et al. (2003) Proc. Natl. Acad. Sci. USA 100:4173.
  14. Arakawa, H. (2004) Nat. Rev. Cancer 4:978.
  15. Oksala, N. et al. (2013) Circ. Cardiovasc. Genet. 6:579.
  16. Ranganathan, P. et al. (2014) J. Am. Soc. Nephrol. 25:239.

Long Name

Netrin Receptor UNC5B

Alternate Names

UNC5B

Entrez Gene IDs

219699 (Human); 60630 (Rat)

Gene Symbol

UNC5B

UniProt

Q8IZJ1

Additional UNC5H2/UNC5B Products

Product Documents for Recombinant Human UNC5B Protein Fc Chimera, CF

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Product Specific Notices for Recombinant Human UNC5B Protein Fc Chimera, CF

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