Recombinant Human Wnt/RSPO3 Agonist Protein, CF

R&D Systems, part of Bio-Techne | Catalog # BT-WRSP3

R&D Systems, part of Bio-Techne
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Key Product Details

Source

CHO

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Wnt/RSPO3 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Wnt/RSPO3 protein
Proprietary

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

SDS-PAGE

83-98 kDa, under reducing conditions.

Activity

Measured by its ability to activate TCF reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 7.50-90.0 ng/mL.

Scientific Data Images for Recombinant Human Wnt/RSPO3 Agonist Protein, CF

Recombinant Human Wnt/RSPO3 Agonist Protein Bioactivity.

Recombinant Human Wnt/RSPO3 Agonist (Catalog # BT-WRSP3) activates TCF reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 7.50-90.0 ng/mL.

Recombinant Human Wnt/RSPO3 Agonist Protein SDS-PAGE.

2 μg/lane of Recombinant Human Wnt/RSPO3 Agonist Protein (Catalog # BT-WRSP3) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 83-98 kDa under reducing conditions.

Formulation, Preparation and Storage

BT-WRSP3
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Wnt/RSPO3

Wnt signaling is known to promote self-renewal of adult stem cells. R-Spondins amplify Wnt signaling in adult stem cells by binding to LGR5 receptors resulting in increased levels of Wnt receptor localization at the membrane surface. Many current organoid protocols include addition of Wnt-3a and R-Spondin proteins to the media for optimal adult stem cell maintenance and health of organoids. Fusion proteins known known as Wnt Surrogates have been described that have enhanced solubility when compared to the widely used Wnt-3a protein and mirror the activities of Wnt signaling.1 R&D Systems has constructed recombinant fusion proteins designed to bind elements of the Wnt receptor system including LGR-5, Frizzled, and LRP-5/6. These can initiate canonical Wnt signaling and are designed to simplify cell culture protocols by substituting for conditioned media or the addition of recombinant Wnt and R-Spondin.

References

  1. Janda et al. (2017) Nature 545:234.

Long Name

Wnt/RSPO3

Alternate Names

R-spondin 3 homolog, roof plate-specific spondin-3

Entrez Gene IDs

84870 (Human); 72780 (Mouse); 498997 (Rat)

Gene Symbol

Q9BXY4

Additional Wnt/RSPO3 Products

Product Documents for Recombinant Human Wnt/RSPO3 Agonist Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Wnt/RSPO3 Agonist Protein, CF

For research use only

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