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Recombinant Mouse Aminopeptidase P2/XPNPEP2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 2494-ZN

R&D Systems, part of Bio-Techne
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2494-ZN-010

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Enzyme Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Aminopeptidase P2/XPNPEP2 protein
Tyr22-Ala650 & Pro23-Ala650, both with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Tyr22 & Pro23

Predicted Molecular Mass

73 kDa

SDS-PAGE

93 kDa, reducing conditions

Activity

Measured by its ability to cleave the fluorogenic peptide substrate, H-Lys(2-Aminobenzoyl)-Pro-Pro-p-Nitroanilide (K(Abz)PP-pNA).
The specific activity is >175 pmol/min/µg, as measured under the described conditions.

Formulation, Preparation and Storage

2494-ZN
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Aminopeptidase P2/XPNPEP2

The mouse XPNPEP2 gene encodes Aminopeptidase P2 (APP2), which is also known as X-prolyl Aminopeptidase 2 or membrane bound Aminopeptidase P (1, 2). It is a member of the M24 family of metalloproteases, which also contains methionine aminopeptidases, X-Pro dipeptidase, Aminopeptidase P1, Aminopeptidase P homolog, proliferation-associated protein 1, and suppressor of Ty homolog or chromatin-specific transcription elongation factor large subunit (3). Mammalian APP2 are predicted to be GPI-anchored membrane proteases and their biological functions have been reviewed (4). Mouse APP2 is expressed in many adult tissues and in embryos (5). The purified rmAPP2 corresponds to the ectodomain and is shown here to be an active aminopeptidase, removing an N-terminal amino acid from a peptide that contains a Pro residue at the second position.

References

  1. Kawai, J. et al. (2001) Nature 409:685.
  2. Shibata, K. et al. (2000) Genome Res. 10:1757.
  3. Barrett, A.J. et al. (2004) Handbook of Proteolytic Enzymes, Elsevier Academic Press, San Diego.
  4. Simmons, W.H. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) pp. 934, Elsevier Academic Press, San Diego.
  5. Ersahin C. et al. (2003) Arch. Biochem. Biophys. 417:131.
 

Alternate Names

XPNPEP2

Entrez Gene IDs

7512 (Human); 170745 (Mouse)

Gene Symbol

XPNPEP2

UniProt

Additional Aminopeptidase P2/XPNPEP2 Products

Product Documents for Recombinant Mouse Aminopeptidase P2/XPNPEP2 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse Aminopeptidase P2/XPNPEP2 Protein, CF

The purchase of this product conveys to the buyer the limited, non-exclusive, non-transferable right (without the right to resell, repackage, or further sublicense) to use these reagents for non-commercial research purposes only. No other license is granted to the buyer whether expressly, by implication, by estoppel or otherwise. In particular, the purchase of this product does not include nor carry any right or license to use, develop, or otherwise exploit this product commercially, which includes without limitation, provision of services to a third party, generation of commercial databases, clinical diagnostics or therapeutics, or drug development. This Product is manufactured under a license to U.S. Patent Nos. 6,399,349 and 7,273,718.

Any party desiring rights under this patent should contact Ryogen LLC, Montebello Park, 75 Montebello Road, Suffern, NY 10901.

For research use only

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