Recombinant Mouse Aminopeptidase PILS/ARTS1 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 2500-ZN
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse Aminopeptidase PILS/ARTS1 protein
Ser27-Leu930, with a C-terminal 10-His tag
Ser27-Leu930, with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ser27
Predicted Molecular Mass
105 kDa
SDS-PAGE
111 kDa, reducing conditions
Activity
Measured by its ability to cleave the fluorogenic peptide substrate, Leu-AMC.
The specific activity is >100 pmol/min/µg, as measured under the described conditions.
The specific activity is >100 pmol/min/µg, as measured under the described conditions.
Reviewed Applications
Read 1 review rated 5 using 2500-ZN in the following applications:
Formulation, Preparation and Storage
2500-ZN
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Aminopeptidase PILS/ARTS1
The mouse ARTS1 gene encodes aminopeptidase PILS (Puromycin-Insensitive Leucyl-Specific), which is also known as adipocyte-derived leucine aminopeptidase, type 1 tumor necrosis factor receptor shedding aminopeptidase regulator and ERAAP (the aminopeptidase associated with antigen processing in the endoplasmic reticulum) (1-3). The deduced amino acid sequence of mouse ARTS1 consists of a signal peptide and a large ectodomain. Widely expressed and releasing an N‑terminal amino acid (Leu), the enzyme may play a role in many processes such as antigen processing and angiogenesis.
References
- Miyashita, H. et al. (2002) Blood 99:3241.
- Serwold, T. et al. (2002) Nature 419:480.
- Schomburg, L. (2004) in Handbook of Proteolytic Enzymes. Barrett, et al. eds. p. 311, Academic Press, San Diego.
Long Name
Aminopeptidase Puromycin-insensitive Leucyl-specific
Alternate Names
A-LAP, ARTS1, ERAAP1, ERAP1, PILS-AP
Gene Symbol
ERAP1
UniProt
Additional Aminopeptidase PILS/ARTS1 Products
Product Documents for Recombinant Mouse Aminopeptidase PILS/ARTS1 Protein, CF
Product Specific Notices for Recombinant Mouse Aminopeptidase PILS/ARTS1 Protein, CF
For research use only
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