Recombinant Mouse Angiopoietin-like Protein 4/ANGPTL4, CF

R&D Systems, part of Bio-Techne | Catalog # 9797-AN

C-Terminal Fragment
R&D Systems, part of Bio-Techne
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Key Product Details

Source

NS0

Accession #

Q9Z1P8

Conjugate

Unconjugated

Applications

Bioactivity

View all Angiopoietin-like Protein 4/ANGPTL4 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Angiopoietin-like Protein 4/ANGPTL4 protein
Leu169-Ser410, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu169

Predicted Molecular Mass

28 kDa

SDS-PAGE

36-43 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse Angiopoietin‑like Protein 4/ANGPTL4 is immobilized at 1 μg/mL, 100 μL/well, the concentration of LILRB2/CD85d/ILT4 Fc Chimera (Catalog # 2078-T4) that produces 50% of the optimal binding response is 0.09-0.54 μg/mL.

Scientific Data Images for Recombinant Mouse Angiopoietin-like Protein 4/ANGPTL4, CF

Recombinant Mouse Angiopoietin-like Protein 4/ANGPTL4 Binding Activity

Recombinant Mouse Angiopoietin-like Protein 4/ANGPTL4 Binding Activity

When Recombinant Mouse Angiopoietin-like Protein 4/ANGPTL4 (Catalog# 9797-AN) is immobilized at 1 µg/mL, 100 µL/well, Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera (Catalog # 2078-T4) binds with an ED50 of 0.09-0.54 µg/mL.

Formulation, Preparation and Storage

9797-AN
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Angiopoietin-like Protein 4/ANGPTL4

Angiopoietin-like 4 (ANGPTL4), also known as FIAF, FARP, and PGAR, is a 55 kDa glycoprotein secreted by the liver and fat tissue. It is structurally related to the angoipoietins and contains an N-terminal coiled coil domain and a C-terminal fibrinogen-like domain which can be proteolytically separated in vivo (1). Amino acid 169-410 contains the C-terminal fibrinogen like domain within mouse ANGPTL4, and this domain shares 75% and 97% sequence identity with human and rat homologs, respectively. The coiled coil domain, which is not glycosylated, mediates the formation of variable sized disulfide-linked oligomers (2). This domain directly inhibits lipoprotein lipase, resulting in increased circulating triglyceride levels (3, 4). In humans, the N-terminal fragment and full length ANGPTL4 physically associate with HDL (4). In mouse, however, full length ANGPTL4 associates with HDL, while the N-terminal fragment associates with LDL (4). Circulating ANGPTL4 is decreased in type II diabetics with a subsequent loss of its normal plasma glucose lowering activity (5). Its expression in adipose tissue is induced by fasting and suppressed by feeding (6). In hypoxic areas, ANGPTL4 is induced in both vascular endothelial cells and tumor cells (7, 8). The N-terminal fragment can function as an angiogenesis inhibitor (7, 8). In contrast, the C-terminal fragment modulates cell adhesion through interactions with heparan sulfate proteoglycans, Integrins beta 1 and beta 5, Vitronectin, and Fibronectin, thereby promoting keratinocyte migration and wound healing (7, 9, 10). ANGPTL4 additionally enhances the survival of hematopoietic and mesenchymal stem cells (11, 12). The expression of an undersialylated form of ANGPTL4 in renal podocytes contributes to proteinuria and nephrotic syndrome (13). The immune-inhibitory receptor human leukocyte immunoglobulin-like receptor B2 (LILRB2) and its mouse orthologue paired immunoglobulin-like receptor (PIRB) have been identified as receptors for several ANGPTLs (14) including ANGPTL4.

References

  1. Zhu, P. et al. (2012) Biosci. Rep. 32:211.
  2. Ge, H. et al. (2004) J. Biol. Chem. 279:2038.
  3. Sukonina, V. et al. (2006) Proc. Natl. Acad. Sci. USA 103:17450.
  4. Mandard, S. et al. (2006) J. Biol. Chem. 281:934.
  5. Xu, A. et al. (2005) Proc. Natl. Acad. Sci. USA 102:6086.
  6. Kersten, S. et al. (2000) J. Biol. Chem. 275:28488.
  7. Cazes, A. et al. (2006) Circ. Res. 99:1207.
  8. Le Jan, S. et al. (2003) Am. J. Pathol. 162:1521.
  9. Goh, Y.Y. et al. (2010) Am. J. Pathol. 177:2791.
  10. Goh, Y.Y. et al. (2010) J. Biol. Chem. 285:32999.
  11. Blank, U. et al. (2012) Eur. J. Haematol. 89:198.
  12. Hou, M. et al. (2014) PLoS ONE 9:e85808.
  13. Clement, L.C. et al. (2011) Nat. Med. 17:117.
  14. Zhang, C.C. et al. (2012) Nature 485:656.

Alternate Names

Angiopoietin like Protein 4, ANGPTL4, FIAF, HFARP, PGAR

Entrez Gene IDs

51129 (Human); 57875 (Mouse); 362850 (Rat)

Gene Symbol

ANGPTL4

UniProt

Q9Z1P8

Additional Angiopoietin-like Protein 4/ANGPTL4 Products

Product Documents for Recombinant Mouse Angiopoietin-like Protein 4/ANGPTL4, CF

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Product Specific Notices for Recombinant Mouse Angiopoietin-like Protein 4/ANGPTL4, CF

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