Recombinant Mouse Bcl-xL (minus C-Terminus) Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 878-BC
Key Product Details
Source
E. coli
Accession #
Structure / Form
Monomer and disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Inhibition Activity
Product Specifications
Source
E. coli-derived mouse Bcl-xL protein
Ser2-Arg212, with a C-terminal 6-His tag
Ser2-Arg212, with a C-terminal 6-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
N-terminal Sequence Analysis
Ser2
Predicted Molecular Mass
25 kDa
SDS-PAGE
27 kDa, under reducing and non-reducing conditions
Activity
Measured by its ability to inhibit Caspase-8-cleaved BID-mediated release of cytochrome c from isolated mouse liver mitochondria using the Rat/Mouse Cytochrome c Quantikine ELISA (Catalog # MCTC0) to quantify cytochrome c.
The typical EC50 for Bcl-xL in this assay is 400 nM in the presence 54 nM of Recombinant Human or Mouse BID Caspase-8-cleaved (Catalog # 882-B8 or 883-M8, respectively).
Optimal dilutions should be determined by each laboratory for each application.
The typical EC50 for Bcl-xL in this assay is 400 nM in the presence 54 nM of Recombinant Human or Mouse BID Caspase-8-cleaved (Catalog # 882-B8 or 883-M8, respectively).
Optimal dilutions should be determined by each laboratory for each application.
Formulation, Preparation and Storage
878-BC
| Formulation | Supplied as a 0.2 μm filtered solution in HEPES and KCl. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Bcl-xL
Bcl-xL is a member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability (1, 2). Bcl-xL is an anti‑apoptotic member that prevents release of cytochrome c from the mitochondria intermembrane space into the cytosol. Bcl-xL is present on the outer mitochondrial membrane and is also found on other membranes in some cell types. Natural Bcl-xL contains a carboxyl‑terminal mitochondria targeting sequence. Recombinant Bcl-xL missing the mitochondrial targeting sequence maintains its ability to neutralize pro‑apoptotic Bcl-2 family members. Neutralization by Bcl-xL appears to be through binding the BH3 region of pro‑apoptotic Bcl-2 family members. This activity does not require the mitochondrial targeting sequence.
References
- Gross, A. et al. (1999) Genes and Develop. 13:1899.
- Kroemer, G. (1997) Nature Med. 3:614.
Long Name
B Cell Lymphoma/Leukemia x Long Form
Alternate Names
BclxL
Gene Symbol
BCL2L1
UniProt
Additional Bcl-xL Products
Product Documents for Recombinant Mouse Bcl-xL (minus C-Terminus) Protein, CF
Product Specific Notices for Recombinant Mouse Bcl-xL (minus C-Terminus) Protein, CF
For research use only
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