Recombinant Mouse BMP-6 Protein

R&D Systems, part of Bio-Techne | Catalog # 6325-BM

R&D Systems, part of Bio-Techne
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Carrier Free
6325-BM-020/CF
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With Carrier
6325-BM-020
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Key Product Details

Source

CHO

Accession #

P20722

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all BMP-6 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse BMP-6 protein
Ser372-His510

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser372

Predicted Molecular Mass

15.5 kDa

SDS-PAGE

17-21 kDa, reducing conditions

Activity

Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351.
The ED50 for this effect is 0.1-0.4 µg/mL.

Reviewed Applications

Read 1 review rated 4 using 6325-BM in the following applications:

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 6325-BM
Formulation Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
Reconstitution Reconstitute at 200 μg/mL in 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 6325-BM/CF
Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution Reconstitute at 200 μg/mL in 4mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-6

Bone Morphogenetic Protein 6 (BMP‑6), also known as Vgr-1, is one of at least 15 structurally and functionally related BMPs which are members of the transforming growth factor  beta (TGF-beta) superfamily. Mouse BMP‑6 is synthesized as a 510 amino acid (aa) precursor protein that is cleaved at the dibasic cleavage site (RxxR) to release the 18 kDa C‑terminal mature protein. Biologically active BMP‑6 consists of a disulfide-linked homodimer of the mature proteins (1, 2). Mature mouse BMP‑6 shares 96% and 98% aa sequence identity with human and rat BMP‑6, respectively. Cellular responses to BMP‑6 are mediated by hetero-oligomeric complexes of type I (Activin RIA/ALK-2 and BMPR-IA/ALK-3) and type II (Activin RIIA and BMPR-II) serine/threonine kinase receptors (3 - 5). Glycosylation of BMP‑6 is required for its interaction with Activin RIA (6). BMP‑6 induces the expression of Noggin and is subsequently antagonized by Noggin (7, 8). BMP‑6 induces a wide range of cellular responses. It promotes osteoblast differentiation from mesenchymal stem cells (5), chondrocyte maturation (9), Ang II-induced aldosterone production in the adrenal cortex (3), hormone production and responsiveness in ovarian granulosa cells (10), iNOS and TNF-alpha production in macrophages (4), the cell death of B cells (8), and neurite outgrowth (11). BMP‑6 expression is induced in astrocytes surrounding sites of brain injury where it functions as a neuroprotectant (11, 12). BMP‑6 is upregulated during prostate cancer progression and promotes tumor cell metastasis to bone (13). Through interactions with the BMP coreceptor RGM‑C/Hemojuvelin, BMP‑6 plays an important role in iron homeostasis by promoting Hepcidin expression and preventing serum iron overload (14, 15).

References

  1. Chen, D. et al. (2004) Growth Factors 22:233.
  2. Lyons, K. et al. (1989) Proc. Natl. Acad. Sci. 86:4554.
  3. Inagaki, K. et al. (2006) Endocrinology 147:2681.
  4. Hong, J.H. et al. (2008) Immunology 128:e442.
  5. Lavery, K. et al. (2008) J. Biol. Chem. 283:20948.
  6. Saremba, S. et al. (2008) FEBS J. 275:172.
  7. Haudenschild, D.R. et al. (2004) Cancer Res. 64:8276.
  8. Kersten, C. et al. (2005) BMC Immunol. 6:9.
  9. Grimsrud, C.D. et al. (1999) J. Bone Miner. Res. 14:475.
  10. Shi, J. et al. (2009) Fertil. Steril. 92:1794.
  11. Yabe, T. et al. (2002) J. Neurosci. Res. 68:161.
  12. Zhang, Z. et al. (2006) Neuroscience 138:47.
  13. Dai, J. et al. (2005) Cancer Res. 65:8274.
  14. Meynard, D. et al. (2009) Nat. Genet. 41:478.
  15. Andriopoulos, B. Jr. et al. (2009) Nat. Genet. 41:482.

Long Name

Bone Morphogenetic Protein 6

Alternate Names

BMP6, Vgr-1

Entrez Gene IDs

654 (Human); 12161 (Mouse); 25644 (Rat)

Gene Symbol

BMP6

UniProt

P20722 (Human)

Additional BMP-6 Products

Product Documents for Recombinant Mouse BMP-6 Protein

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS 6325-BM (with carrier)
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Product Specific Notices for Recombinant Mouse BMP-6 Protein

For research use only

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