Recombinant Mouse CEACAM-5/CD66e His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10404-CM

R&D Systems, part of Bio-Techne
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10404-CM-050
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Key Product Details

Source

NS0

Accession #

Q3UKK2.1

Conjugate

Unconjugated

Applications

Bioactivity

View all CEACAM-5/CD66e Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse CEACAM-5/CD66e protein
Gln35-Glu947, with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln35

Predicted Molecular Mass

103 kDa

SDS-PAGE

115-135 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse CEACAM-5/CD66e His-tag (Catalog # 10404-CM) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Mouse Galectin-4  (Catalog # 2128-GA) binds with an ED50 of 0.1-0.9 μg/mL.

Scientific Data Images for Recombinant Mouse CEACAM-5/CD66e His-tag Protein, CF

Recombinant Mouse CEACAM-5/CD66e His-tag Protein Binding Activity

Recombinant Mouse CEACAM-5/CD66e His-tag Protein Binding Activity

When Recombinant Mouse CEACAM/CD66e His-tag (10404-CM) is immobilized at 0.5 μg/mL (100 μL/well), Recombinant Mouse Galectin-4 (2128-GA) binds with an ED50 of 0.1-0.9 μg/mL.
Recombinant Mouse CEACAM-5/CD66e His-tag Protein SDS-PAGE

Recombinant Mouse CEACAM-5/CD66e His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Mouse CEACAM-5/CD66e His-tag (10404-CM) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 115-135 kDa.

Formulation, Preparation and Storage

10404-CM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 400 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CEACAM-5/CD66e

CEACAM-5, also known as CEA, CD66e and Psg30, belongs to the large family of CEACAM and pregnancy specific glycoproteins. CEACAM family members are highly glycosylated with varying arrangements of IgV-like and IgC-like regions in their extracellular domains (ECD) and can be expressed as transmembrane, glycophosphatidyl inositol (GPI) linked or soluble proteins (1-3). CEACAM-5 consists of an N-terminal Ig-like V-set domain followed by six Ig-like C2-set domains and a GPI anchor (2, 4, 5). While the mature ECD of mouse CEACAM-5 shares 26% amino acid identity with human CEACAM-5, it remains unclear if these molecules are direct orthologs (6). CEACAM-5, expressed primarily by epithelial cells, functions as a calcium-independent adhesion molecule through homophilic and heterophilic interactions with CEACAM-1 (1, 7). CEACAM-5 is restricted to the apical face of intestinal epithelial cells in the adult but is more diffuse during embryonic development and in tumors (8). This is consistent with a role in the development and maintenance of epithelial architecture. CEACAM-5 is up-regulated in a wide variety of human tumors, promoting tumor cell migration, invasion, adhesion, and metastasis, and has been used as a cancer marker (8, 9). It also contributes to tumor formation by maintaining cellular proliferation in the presence of differentiation stimuli, and by blocking apoptosis following loss of ECM anchorage (anoikis) (10, 11). The GPI anchoring of CEACAM-5 can be released by GPI-PLD, resulting in a soluble molecule that also promotes tumor metastasis (12). Cell surface expression of CEACAM-5 on tumor cells prevents the adhesion of CEACAM-1 expressing NK cells and provides protection from NK-mediated lysis (8). CEACAM-5 was shown to bind galectin-4 and by surface plasmon resonance and coimmunoprecipitated with galectin-4 in human colon adenocarcinoma LS174T cell lysates (13). CEACAM-5 also binds a subset of Neisseria opacity proteins (Opa) and E. coli adhesion proteins (14, 15). These interactions trigger clustering of the lipid raft-localized CEACAM-5 to sites of pathogen contact (15, 16).

References

  1. Zebhauser, R. et al. (2005) Genomics 86:566.
  2. Hammarstrom, S. (1999) Semin. Cancer Biol. 9:67.
  3. Chan, et al. (2007) Curr Oncol. 14:70.
  4. Schrewe H. et al. (1990) Mol. Cell. Biol. 10:2738.
  5. Hefta, S.A. et al. (1988) Proc. Natl. Acad. Sci. 85:4648.
  6. Hance KW et al. Mutat Res. (2005) 576:132.
  7. Stern, N. et al. (2005) J. Immunol. 174:6692.
  8. Beauchemin N. et al. (2013) Cancer Metastasis Rev. 32:3.
  9. Blumenthal, R.D. et al. (2005) Cancer Res. 65:8809.
  10. Screaton, R.A. et al. (1997) J. Cell Biol. 137:939.
  11. Ordonez, C. et al. (2000) Cancer Res. 60:3419.
  12. Yamamoto, Y. et al. (2005) Biochem. Biophys. Res. Commun. 333:223.
  13. Ideo H. et al. (2005) J Biol Chem. 280:4730.
  14. Martin, J.N. et al. (2016) Biochemistry. 55:4286
  15. Berger, C.N. et al. (2004) Mol. Microbiol. 52:963.
  16. Tchoupa, A.K. et al. (2014) Cell Commun Signal. 12:27.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 5

Alternate Names

CD66e, CEA, CEACAM5

Entrez Gene IDs

1048 (Human); 73250 (Mouse); 102128791 (Cynomolgus Monkey)

Gene Symbol

CEACAM5

UniProt

Q3UKK2.1

Additional CEACAM-5/CD66e Products

Product Documents for Recombinant Mouse CEACAM-5/CD66e His-tag Protein, CF

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Product Specific Notices for Recombinant Mouse CEACAM-5/CD66e His-tag Protein, CF

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