Recombinant Mouse E-Cadherin Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8875-EC

R&D Systems, part of Bio-Techne
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8875-EC-050
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Key Product Details

Source

NS0

Accession #

P09803

Conjugate

Unconjugated

Applications

Bioactivity

View all E-Cadherin Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse E-Cadherin protein
Asp157-Val709, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp157

Predicted Molecular Mass

62 kDa

SDS-PAGE

75-87 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of the MCF-7 human breast cancer cells.
The ED50 for this effect is 0.25-1 μg/mL.

Reviewed Applications

Read 1 review rated 5 using 8875-EC in the following applications:

Formulation, Preparation and Storage

8875-EC
Formulation Lyophilized from a 0.2 μm filtered solution in MES, NaCl and CaCl2.
Reconstitution
Reconstitute at 250 μg/mL in water.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: E-Cadherin

E-Cadherin/Cadherin-1, also known as Uvomorulin in the mouse and rat, is a 120 kDa member of the Cadherin family of cell surface glycoproteins that mediate cell adhesion (1). Mouse E-Cadherin shares 92% and 81% amino acid sequence identity with the rat and human proteins, respectively (2). It is a single-pass transmembrane protein that mediates calcium-dependent epithelial cell adhesion. E-Cadherin has five extracellular EC domains that form homophilic cis-clusters between adjacent epithelial cells and trans-clusters within the same cell. E-Cadherin clusters are critical components of adherens junctions between epithelial cells and act in the formation and maintenance of the epithelial cell barrier (3, 4). The intracellular domain of E-Cadherin binds to the Catenin cytoskeletal complex, which includes p120 Catenin, beta-Catenin, alpha-Catenin, and Vinculin. E-Cadherin expression is critical for epithelial tissue homeostasis. Decreased E-Cadherin is associated with physiological and pathological epithelial-to-mesenchymal transition and cell migration, and E-Cadherin loss contributes to cancer metastasis (5). The extracellular E-Cadherin N-terminal domain can be cleaved by several proteases and is released as a soluble factor that enhances cancer cell motility and EGFR-dependent survival and proliferation (6).

References

  1. Gumbiner, B.M. (2005) Nat. Rev. Mol. Cell Biol. 6:622.
  2. Nagafuchi, A. et al. (1987) Nature 329:341.
  3. Guillot, C. and T. Lecuit (2013) Science 340:1185.
  4. Tian, X. et al. (2011) J. Biomed. Biotechnol. 2011:567305.
  5. Stemmler, M.P. (2008) Mol. Biosyst. 4:835.
  6. David, J.M. and A.K. Rajasekaran (2012) Cancer Res. 72:2917.

Alternate Names

Arc-1, CAD1, Cadherin-1, CD324, CDH1, Cell-CAM120/80, ECAD, ECadherin, L-CAM, Uvomorulin

Entrez Gene IDs

999 (Human); 12550 (Mouse); 83502 (Rat)

Gene Symbol

CDH1

UniProt

P09803

Additional E-Cadherin Products

Product Documents for Recombinant Mouse E-Cadherin Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse E-Cadherin Protein, CF

For research use only

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