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Recombinant Mouse Flt-3/Flk-2 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 768-F3

R&D Systems, part of Bio-Techne
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768-F3-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Flt-3/Flk-2 protein
Mouse Flt-3
(Asn28-Ser544)
Accession # Q00342
IEGRMD Human IgG1
(Pro100-Lys330)
6-His tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asn28

Predicted Molecular Mass

85 kDa (monomer)

SDS-PAGE

116 kDa, reducing conditions

Activity

Measured by its ability to inhibit Flt-3 Ligand-induced proliferation of BaF3 mouse pro-B cells transfected with Flt-3.
The ED50 for this effect is 0.05-0.25 µg/mL in the presence of recombinant mouse Flt-3 Ligand at 10 ng/mL.

Formulation, Preparation and Storage

768-F3
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Flt-3/Flk-2

The Flt-3 (fms-like tyrosine kinase) receptor, also named Flk-2 (fetal liver kinase) and Stk-1 (stem cell tyrosine kinase), is a member of the class III subfamily of receptor tyrosine kinases. This familay includes KIT, the receptor for SCF, and C-FMS, the receptor for M-CSF. The extracellular region of these receptors contains five immunoglobulin-like domains and the intracellular region contains a split kinase domain. Mouse Flt-3 cDNA encodes a 992 amino acid (aa) residue type I membrane protein with a 27 aa residue signal peptide, a 517 aa extracellular domain with 10 potential N-linked glycosylation sites, a 20 aa residue transmembrane domain and a 428 aa residue cytoplasmic domain. Mouse Flt-3 shares 85% amino acid sequence identity with human Flt-3. Flt-3 expression has been detected in various tissues, including placenta, gonads, and tissues of nervous and hematopoietic origin. Among hematopoietic cells, the expression of Flt-3 was found to be restricted to the highly enriched stem/progenitor cell populations. The ligand for Flt-3 (FL) has been identified to be a transmembrane protein with structural homology to M-CSF and SCF. Recombinant soluble Flt-3/Fc chimeric protein has been shown to bind FL with high affinity and is a potent FL antagonist.

References

  1. Rosnet, O. et al. (1996) Acta. Haemato. 95:218.
  2. Drexler, H.G. (1996) Leukemia 10:588.

Long Name

fms-like Tyrosine Kinase 3

Alternate Names

CD135, Flk-2, Flt3, STK-1

Entrez Gene IDs

2322 (Human); 14255 (Mouse)

Gene Symbol

FLT3

UniProt

Additional Flt-3/Flk-2 Products

Product Documents for Recombinant Mouse Flt-3/Flk-2 Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse Flt-3/Flk-2 Fc Chimera Protein, CF

For research use only

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