Recombinant Mouse G-CSFR alpha/CD114 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6039-GR

R&D Systems, part of Bio-Techne
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6039-GR-050
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Key Product Details

Source

NS0

Accession #

P40223

Structure / Form

Noncovalently-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all G-CSFR/CD114 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse G-CSF R/CD114 protein
Met1-Asp626, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Cys26

Predicted Molecular Mass

68.2 kDa (monomer)

SDS-PAGE

100-110 kDa, reducing conditions

Activity

Measured by its ability to inhibit the G-CSF-induced proliferation of NFS-60 mouse myelogenous leukemia lymphoblast cells.
The ED50 for this effect is 0.02-0.12 µg/mL in the presence of 0.125 ng/mL of recombinant mouse G-CSF (Catalog # 414-CS).

Formulation, Preparation and Storage

6039-GR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: G-CSFR/CD114

Granulocyte colony stimulating factor (G-CSF) is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic and granulocyte lineage (1). G-CSF plays an important role in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis. Cell activation by G‑CSF is mediated by granulocyte colony stimulating factor receptor alpha (G-CSF R; also CD114), a 95 ‑ 105 kDa type I transmembrane protein and member of the cytokine receptor superfamily, type I cytokine receptor family, and type 2 subfamily of receptor proteins. Mouse G‑CSF R is synthesized as an 837 amino acid (aa) precursor that contains a 25 aa signal sequence, a 601 aa extracellular domain (ECD), a 24 aa transmembrane region, and a 187 aa cytoplasmic tail. The ECD contains one Ig-like C2-type domain, five fibronectin type-III domains, and 11 potential sites for N-linked glycosylation. Within the ECD there is also a WSXWS motif (aa 319 ‑ 323) that is necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding (2). Also, within the cytoplasmic domain there is a Box 1 motif which is required for JAK interaction and/or activation (1). Mouse G‑CSF R shares 63% aa sequence identity with human G‑CSF R. G-CSF R is expressed in mature neutrophils, neutrophilic precursors, myeloid leukemia cells, and placenta (1). Mutations have been found in the gene encoding G-CSF R in some patients with severe congenital neutropenia (1). These mutations typically lead to a truncation in the cytoplasmic domain of the G-CSF R leading to maturation arrest of neutrophilic precursors in the bone marrow and neutropenia in peripheral blood (3). Binding of G-CSF to its receptor induces dimerization or oligomerization of the receptor activating cytoplasmic tyrosine kinases (2). Signal transduction from pathways that involve Janus tyrosine kinases/signal transducer and activator of transcription proteins (Jak1, Jak2, and Tyk2/STAT3 and STATG), src-related protein tyrosine kinases (Lyn and Syk), Ras/MAP kinase, and phosphatidylinositol have been reported to be activated upon G-CSF stimulation (4).

References

  1. Ward, A.C. (2007) Front. Biosci. 12:608.   
  2. Layton, J.E. and N.E. Hall (2006) Front. Biosci. 11:3181.
  3. Mitsui, T. et al. (2003) Blood 101:2990.
  4. Nicola, N.A. in Cytokine Reference, 2001, Oppenheim, J.J. and M. Feldmann, eds. Academic Press p.1935.

Long Name

Granulocyte Colony Stimulating Factor Receptor

Alternate Names

CD114, CSF3R, Csfgr, G-CSF R, GCSFR

Entrez Gene IDs

1441 (Human); 12986 (Mouse)

Gene Symbol

CSF3R

UniProt

P40223

Additional G-CSFR/CD114 Products

Product Documents for Recombinant Mouse G-CSFR alpha/CD114 Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Mouse G-CSFR alpha/CD114 Protein, CF

For research use only

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