Recombinant Mouse GDF-5 Protein
R&D Systems, part of Bio-Techne | Catalog # 853-G5
Key Product Details
Source
E. coli
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
E. coli-derived mouse GDF-5/BMP-14 protein
Ala376-Arg495, with and without an N-terminal Met
Ala376-Arg495, with and without an N-terminal Met
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met and Ala376
Predicted Molecular Mass
13.7 kDa (monomer)
Activity
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351.
The ED50 for this effect is 0.2-1.2 µg/mL.
The ED50 for this effect is 0.2-1.2 µg/mL.
Reviewed Applications
Read 1 review rated 3 using 853-G5 in the following applications:
Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 853-G5
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 150 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 853-G5/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. |
| Reconstitution | Reconstitute at 150 μg/mL in sterile 4 mM HCl. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: GDF-5/BMP-14
Growth Differentiation Factor-5 (GDF-5; also called BMP-14 and CDMP-1) is a member of the BMP family of TGF-beta superfamily proteins (1, 2). GDF-5, -6, and -7 are a defined subgroup of the BMP family (3). GDF-5 is synthesized as a large precursor protein that consists of an N-terminal 19 amino acid (aa) signal sequence, a 362 aa pro region and a 120 aa C-terminal mature peptide. Mature mouse GDF-5 shares 99% aa sequence identity with both mature human and rat GDF-5. GDF-5 signaling is mediated by formation of a heterodimeric complex consisting of a type 1 (BMPR-IB) and a type II (BMPR-II or Activin RII) serine/threonine kinase receptor which results in the phosphorylation and activation of cytosolic Smad proteins (Smad1, 5, and 8) (4, 5). Similar to other BMP family proteins, GDF-5 signaling is antagonized by Noggin (6). GDF-5 is involved in multiple developmental processes including limb generation, cartilage development, joint formation, bone morphogenesis, cell survival, and neuritogenesis (7-11). Exogenous GDF-5 has been reported to promote chondrogenesis, osteogenesis, and angiogenesis in mesenchymal stem cells in vivo and in vitro (12-14). Inhibition of GDF-5 expression or alteration of its signaling can facilitate the development of osteoarthritis (15-18).
References
- Storm, E.E. et al. (1994) Nature 368:639.
- Hotten, G. et al. (1994) Biochem. Biophys. Res. Commun. 204:646.
- Mikic, B. (2004) Ann. Biomed. Eng. 32:466.
- Nishitoh, H. et al. (1996) J. Biol. Chem. 271:21345.
- Mueller, T.D. and J. Nickel (2012) FEBS Lett. 586:1846.
- Bragdon, B. et al. (2011) Cell. Signal. 23:609.
- Sieber, C. et al. (2006) Biol. Chem. 387:451.
- Osorio, C. et al. (2013) Development 140:4751.
- Wood, T.K. et al. (2005) J. Neurosci. Res. 80:759.
- Francis-West, P.H. et al. (1999) Development 126:1305.
- Brunet, L.J. et al. (1998) Science 280:1455.
- Zeng, Q. et al. (2007) Bone 40:374.
- Coleman, C.M. et al. (2013) Stem Cells Dev. 22:1968.
- Cheng, X. et al. (2012) Cells Tissues Organs 196:56.
- Zhang, Y. et al. (2014) Exp. Mol. Med. 46:e79.
- Miyamoto, Y. et al. (2007) Nat. Genet. 39:529.
- Vaes, R.B. et al. (2009) Ann. Rheum. Dis. 68:1754.
- Degenkolbe, E. et al. (2013) PLoS Genet. 9:e1003846.
Long Name
Growth Differentiation Factor 5
Alternate Names
BMP-14, CDMP-1, GDF5
Gene Symbol
GDF5
UniProt
Additional GDF-5/BMP-14 Products
Product Documents for Recombinant Mouse GDF-5 Protein
Product Specific Notices for Recombinant Mouse GDF-5 Protein
For research use only
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