Recombinant Mouse IL-18/IL-1F4 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # B004-5
A New rm IL-18 is Now Available! Competitively priced; Enhanced stability; Lower endotoxin specification; Made in-house!
Key Product Details
Source
E. coli
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
E. coli-derived mouse IL-18/IL-1F4 protein
36-192 aa
36-192 aa
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Predicted Molecular Mass
18 kDa
Activity
Induction of IFN-gamma by Mouse IL-18 receptor transfected KG-1 cell [human myelomonocyte; ATCC CCL246] in response to the recombinant mouse IL-18 was measured using human IFN-gamma ELISA. The activity of lot 040 was as follows:IL-18 final concentration 5ng/mL; IFN-gamma induction 2863 IU/mL and IL-18 final concentration 10 ng/mL; IFN-gamma induction 7312 IU/mL.
Formulation, Preparation and Storage
B004-5
| Formulation | Lyophilized in PBS containing 1% sucrose. |
| Reconstitution | Reconstitute in 250 μL of ice-cold distilled water on ice. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date. |
Background: IL-18/IL-1F4
IL-18 activation is induced by infection or tissue damage and contributes to disease pathology in chronic inflammation (1-3). IL-18 binds to the widely expressed IL-18 R alpha which recruits IL-18 R beta to form the signaling receptor complex (4, 5). Its bioactivity is negatively regulated by interactions with IL-18 binding proteins and virally encoded IL-18BP homologs (6). In the presence of IL-12 or IL-15, IL-18 enhances anti-viral Th1 immune responses by inducing IFN-gamma production and the cytolytic activity of CD8+ T cells and NK cells (7, 8). In the absence of IL-12 or IL-15, however, IL-18 promotes production of the Th2 cytokines IL-4 and IL-13 by CD4+ T cells and basophils (9, 10). In the presence of IL-1 beta or IL-23, IL-18 induces the antigen-independent production of IL-17 by gamma delta T cells and CD4+ T cells (11). IL-18 also promotes myeloid dendritic cell maturation and triggers neutrophil respiratory burst (12, 13). In cancer, IL-18 exhibits diverse activities including enhancing anti-tumor immunity, inhibiting or promoting angiogenesis, and promoting tumor cell metastasis (14). Mature mouse IL-18 shares 63% and 91% amino acid sequence identity with mouse and rat IL-18, respectively (15).
References
- Dinarello, C.A. et al. (2013) Front. Immunol. 4:289.
- Smith, D.E. (2011) J. Leukoc. Biol. 89:383.
- Gu, Y. et al. (1997) Science 275:206.
- Torigoe, K. et al. (1997) J. Biol. Chem. 272:25737.
- Cheung, H. et al. (2005) J. Immunol. 174:5351.
- Novick, D. et al. (1999) Immunity 10:127.
- Fehniger, T.A. et al. (1999) J. Immunol. 162:4511.
- Yoshimoto, T. et al. (1998) J. Immunol. 161:3400.
- Yoshimoto, T. et al. (2000) Nat. Immunol. 1:132.
- Kroeger, K.M. et al. (2009) J. Leukoc. Biol. 86:769.
- Lalor, S.J. et al. (2011) J. Immunol. 186:5738.
- Li, J. et al. (2004) Cell. Immunol. 227:103.
- Elbim, C. et al. (2005) Clin. Diagn. Lab. Immunol. 12:436.
- Fabbi, M. et al. (2015) J. Leukoc. Biol. 97:665.
- Okamura, H. et al. (1995) Nature 378:88.
Long Name
Interleukin 18
Alternate Names
IGIF, IL-1F4, IL-1g, IL18
Gene Symbol
IL18
Additional IL-18/IL-1F4 Products
Product Documents for Recombinant Mouse IL-18/IL-1F4 Protein, CF
Product Specific Notices for Recombinant Mouse IL-18/IL-1F4 Protein, CF
For research use only
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