Recombinant Mouse LIFR alpha Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 5990-LR

R&D Systems, part of Bio-Techne
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5990-LR-050
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Key Product Details

Source

NS0

Accession #

P42703

Conjugate

Unconjugated

Applications

Bioactivity

View all LIFR alpha Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse LIF R alpha protein
Leu44-Ser828, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu44

Predicted Molecular Mass

89.4 kDa

SDS-PAGE

125-140 kDa, reducing conditions

Activity

Measured by its ability to inhibit LIF-dependent proliferation of TF-1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323.
The ED50 for this effect is 0.8-4 μg/mL.

Formulation, Preparation and Storage

5990-LR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LIFR alpha

Leukemia Inhibitory Factor Receptor alpha (LIF R alpha), also known as LIFR beta and CD118, is a 190 kDa type I transmembrane protein in the Interleukin-6 receptor family. Members of this family mediate the biological effects of Cardiotrophin-1, CLC, CNTF, IL-6, IL-11, IL-27, and Oncostatin M (1). Mature mouse LIF R alpha consists of a 785 amino acid (aa) extracellular domain (ECD) with two cytokine receptor homology domains, one WSxWS motif, and three fibronectin type III repeats, followed by a 25 aa transmembrane segment and a 239 aa cytoplasmic domain (2, 3). Within the ECD, mouse LIF R alpha shares 73% and 90% aa sequence identity with human and rat LIF R alpha, respectively. Alternative splicing generates a 90 kDa soluble form of the mouse LIF R alpha ECD (4). LIF R alpha binds the pleiotropic cytokine LIF with low affinity, and the soluble isoform retains LIF-binding activity (5). Binding affinity is increased by the ligand-induced association of LIF R alpha with the signal transducing subunit gp130 (6, 7). The LIF R alpha/gp130 receptor complex also transduces Oncostatin M signals, although LIF R alpha alone does not interact with Oncostatin M (6). gp130 associates with different ligand-specific receptors to form signaling receptor complexes for the other IL-6 family ligands (1). The CNTF receptor is a ternary complex that contains CNTF R alpha and gp130 as well as LIF R alpha (8, 9). LIF R alpha is widely expressed, and LIF induces the proliferation, differentiation, and activation of cells in many tissues (10, 11). In particular, LIF R alpha plays an important role in several aspects of early pregnancy such as blastocyst implantation in the uterus (4, 12 - 14).

References

  1. Muller-Newen, G. (2003) Science STKE 2003:pe40.
  2. Gearing, D.P. et al. (1991) EMBO J. 10:2839.
  3. Tomida, M. et al. (1994) J. Biochem. 115:557.
  4. Owczarek, C.M. et al. (1996) J. Biol. Chem. 271:5495.
  5. Layton, M.J. et al. (1992) Proc. Natl. Acad. Sci. 89:8616.
  6. Gearing, D.P. et al. (1992) Science 255:1434.
  7. Giese, B. et al. (2005) J. Cell Sci. 118:5129.
  8. Ip, N.Y. et al. (1992) Cell 69:1121.
  9. Davis, S. et al. (1993) Science 260:1805.
  10. Metcalf, D (2003) Stem Cells 21:5.
  11. Kubota, Y. et al. (2008) J. Clin. Invest. 118:2393.
  12. Paiva, P. et al. (2009) Cytokine Growth Factor Rev. 20:319.
  13. Stewart, C.L. et al. (1992) Nature 359:76.
  14. Cheng, J.-G. et al. (2001) Proc. Natl. Acad. Sci. 98:8680.

Long Name

Leukemia Inhibitory Factor Receptor alpha

Alternate Names

CD118, LIF R alpha, LIF R beta, LIFR, LIFRa

Entrez Gene IDs

3977 (Human); 16880 (Mouse); 81680 (Rat); 102131906 (Cynomolgus Monkey)

Gene Symbol

LIFR

UniProt

P42703

Additional LIFR alpha Products

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Product Specific Notices for Recombinant Mouse LIFR alpha Protein, CF

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