Recombinant Mouse Nephrin Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 3159-NN
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse Nephrin protein
Gln37-Thr1049, with a C-terminal 6-His tag
Gln37-Thr1049, with a C-terminal 6-His tag
Purity
>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
No results obtained: Gln37 predicted
Predicted Molecular Mass
110 kDa
SDS-PAGE
130-145 kDa, reducing conditions
Activity
Measured by the ability of the immobilized protein to support the adhesion of MS-1 mouse pancreatic islet endothelial cells.
When 5 x 104 cells/well are added to rmNephrin coated plates (10 µg/mL, 100 µL/well), >60% will adhere after 90 minutes at 37° C.
When 5 x 104 cells/well are added to rmNephrin coated plates (10 µg/mL, 100 µL/well), >60% will adhere after 90 minutes at 37° C.
Formulation, Preparation and Storage
3159-NN
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 100 μg/mL in sterile PBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Nephrin
Nephrin is a 185 kDa type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily (1). Mature mouse Nephrin consists of a 1042 amino acid (aa) extracellular domain (ECD) with eight Ig-like C2-set domains and one fibronectin type III domain, a 22 aa transmembrane segment, and a 156 aa cytoplasmic tail (2, 3). Within the ECD, mouse Nephrin shares 84% and 95% aa sequence identity with human and rat Nephrin, respectively. Usage of the alternate exon 1B results in a distinct N-terminal sequence that lacks a clearly defined signal peptide cleavage site (4). Nephrin is expressed primarily on podocytes in the renal glomerulus and to a lesser extent in the brain and pancreas (3, 5). The 1B isoform is not expressed in the kidney (4). Nephrin localizes to intercellular junctions between podocyte foot processes where it functions as a homophilic adhesion molecule (2, 6). Nephrin is required for formation and maintenance of the slit diaphragm between these processes (7). It associates with Neph1, podicin, P-cadherin, and multiple scaffolding proteins which couple it to the actin cytoskeleton (8 - 12). Nephrin expression is required for the anti-apoptotic effect of VEGF on podocytes as well as for the ability of podocytes to upregulate Glut1 and Glut4 glucose transporters in response to insulin (13, 14). Nephrin downregulation contributes to diabetic nephropathy, and Nephrin mutations underlie the lethal congenital nephritic syndrome NPHS1 (5, 15).
References
- Kawachi, H. et al. (2006) Nephrology 11:274.
- Holzman, L.B. et al. (1999) Kidney Int. 56:1481.
- Putaala, H. et al. (2000) J. Am. Soc. Nephrol. 11:991.
- Beltcheva, O. et al. (2003) J. Am. Soc. Nephrol. 14:352.
- Putaala, H. et al. (2001) Hum. Mol. Genet. 10:1.
- Khoshnoodi, J. et al. (2003) Am. J. Pathol. 163:2337.
- Ruotsalainen, V. et al. (2000) Am. J. Pathol. 157:1905.
- Barletta, G.M. et al. (2003) J. Biol. Chem. 278:19266.
- Huber, T.B. et al. (2001) J. Biol. Chem. 276:41543.
- Lehtonen, S. et al. (2004) Am. J. Pathol. 165:923.
- Lehtonen, S. et al. (2005) Proc. Natl. Acad. Sci. 102:9814.
- Verma, R. et al. (2006) J. Clin. Invest. 116:1346.
- Foster, R.R. et al. (2005) Am. J. Physiol. Renal Physiol. 288:F48.
- Coward, R.J. et al. (2007) Diabetes 56:1127.
- Cooper, M.E. et al. (2002) Semin. Nephrol. 22:393.
Alternate Names
CNF, NPHS1
Gene Symbol
NPHS1
UniProt
Additional Nephrin Products
Product Documents for Recombinant Mouse Nephrin Protein, CF
Product Specific Notices for Recombinant Mouse Nephrin Protein, CF
For research use only
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