Recombinant Mouse Noggin Protein
R&D Systems, part of Bio-Techne | Catalog # 1967-NG
Key Product Details
Source
NS0
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse Noggin protein
| Mouse Noggin (Leu20-Cys232) Accession # P97466 |
IEGRGGGSGGGSGGGS | 10-His tag |
| N-terminus | C-terminus |
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Leu20
Predicted Molecular Mass
26 kDa (monomer)
SDS-PAGE
30-35 kDa, reducing conditions
Activity
Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells.
The ED50 for this effect is 0.06-0.3 µg/mL in the presence of 50 ng/mL of Recombinant Human BMP-4 (Catalog # 314-BP).
The ED50 for this effect is 0.06-0.3 µg/mL in the presence of 50 ng/mL of Recombinant Human BMP-4 (Catalog # 314-BP).
Reviewed Applications
Read 3 reviews rated 4.3 using 1967-NG in the following applications:
Scientific Data Images for Recombinant Mouse Noggin Protein
Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 1967-NG
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 1967-NG/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Noggin
Noggin was originally cloned based on its dorsalizing activity in Xenopus embryos. Mammalian Noggins were subsequently identified and cloned from human, mouse and rat cDNA libraries. Mouse Noggin cDNA encodes a 232 amino acid (aa) residue precursor protein with 19 aa residue putative signal peptide that is cleaved to generate the 213 aa residue mature protein which is secreted as a homodimeric glycoprotein. Noggin is a highly conserved molecule. Mature mouse Noggin shares 99% and 83% aa sequence identity with human and Xenopus Noggin, respectively. Noggin has a complex pattern of expression during embryogenesis. In the adult, Noggin is expressed in the central nervous system and in several adult peripheral tissues such as lung, skeletal muscle and skin. Noggin has been shown to be a high-affinity BMP (bone morphogenetic protein) binding protein that antagonizes BMP bioactivities.
References
- Smith, W.C. and R.M. Harland (1992) Cell 70:829.
- Valenzuela, D.M. et al. (1995) J. Neurosci. 15:6077.
- Brunet, L.J. et al. (1998) Science 280:1455.
Alternate Names
NOG, SYM1, SYNS1, SYNS1A
Gene Symbol
NOG
UniProt
Additional Noggin Products
Product Documents for Recombinant Mouse Noggin Protein
Product Specific Notices for Recombinant Mouse Noggin Protein
For research use only
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