Recombinant Mouse Tie-2 His-tag Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 10487-T2
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse Tie-2 protein
Ala23-Lys744, with a C-terminal 6-His tag
Ala23-Lys744, with a C-terminal 6-His tag
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala23
Predicted Molecular Mass
81 kDa
SDS-PAGE
96-107 kDa, under reducing conditions
Activity
Measured by its binding ability in a functional ELISA.
When Recombinant Mouse Tie-2 His-tag (Catalog # 10487-T2) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Human Angiopoietin-2 His-tag (Catalog # 623-AN) binds with an ED50 of 0.4-3.6 ng/mL.
When Recombinant Mouse Tie-2 His-tag (Catalog # 10487-T2) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Human Angiopoietin-2 His-tag (Catalog # 623-AN) binds with an ED50 of 0.4-3.6 ng/mL.
Scientific Data Images for Recombinant Mouse Tie-2 His-tag Protein, CF
Recombinant Mouse Tie-2 His-tag Protein SDS-PAGE
2 μg/lane of Recombinant Mouse Tie-2 His-tag Protein (Catalog # 10487-T2) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 95-110 kDa.
Formulation, Preparation and Storage
10487-T2
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Tie-2
Tie-1/Tie (tyrosine kinase with Ig and EGF homology domains 1) and Tie-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains, followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis.
Two ligands, angiopoietin-1 (Ang1) and angiopoietin-2 (Ang2), which bind Tie-2 with high-affinity have been identified. Ang2 has been reported to act as an antagonist for Ang1. Mice engineered to overexpress Ang2 or to lack Ang1 or Tie-2 display similar angiogenesis defects.
References
- Partanen, J. and D.J. Dumont (1999) Curr. Top. Microbiol. Immunol. 237:159.
- Takakura, N. et al. (1998) Immunity 9:677.
Long Name
Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains 2
Alternate Names
CD202b, TEK, Tie2
Entrez Gene IDs
Gene Symbol
TEK
UniProt
Additional Tie-2 Products
Product Documents for Recombinant Mouse Tie-2 His-tag Protein, CF
Product Specific Notices for Recombinant Mouse Tie-2 His-tag Protein, CF
For research use only
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