Recombinant Mouse TIM-1/KIM-1/HAVCR Isoform A Fc Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10699-TM

R&D Systems, part of Bio-Techne
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10699-TM-050
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Key Product Details

Source

NS0

Accession #

NP_599009.2

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all TIM-1/KIM-1/HAVCR Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse TIM-1/KIM-1/HAVCR protein
Mouse TIM-1/KIM-1/HAVCR
(Tyr22-Gly237)
Accession # NP_599009.2		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Tyr22

Predicted Molecular Mass

50 kDa

SDS-PAGE

75-85 kDa, under reducing conditions

Activity

Measured in a cell proliferation assay using PHA-stimulated human T lymphoblasts.
The ED50 for this effect is 0.5-4.0 μg/mL..

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse TIM-1/KIM-1/HAVCR Isoform A Fc Chimera (Catalog # 10699-TM) is immobilized at 1 µg/mL (100 µL/well), Recombinant Mouse TIM-4 (Catalog # 2826-TI) binds with an ED50 of 0.8-6.4 µg/mL.

Scientific Data Images for Recombinant Mouse TIM-1/KIM-1/HAVCR Isoform A Fc Protein, CF

Recombinant Mouse TIM-1/KIM-1/HAVCR Isoform A Fc Chimera Protein Bioactivity.

Recombinant Mouse TIM-1/KIM-1/HAVCR Isoform A Fc Chimera (Catalog # 10699-TM) inhibits the proliferation of PHA-stimulated human T lymphoblasts. The ED50 for this effect is 0.5-4.0 μg/mL.

Recombinant Mouse TIM-1/KIM-1/HAVCR Isoform A Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Mouse TIM-1/KIM-1/HAVCR Isoform A Fc Chimera (Catalog # 10699-TM) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 75-85 kDa and 150-170 kDa, respectively.

Formulation, Preparation and Storage

10699-TM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIM-1/KIM-1/HAVCR

TIM‑1 (T cell‑immunoglobulin‑mucin; also KIM‑1 and Tapr) is a 70-80 kDa, type I transmembrane glycoprotein member of the TIM family of immunoglobulin superfamily molecules (1, 2, 3, 4). This gene family is involved in the regulation of Th1 and Th2‑cell‑mediated immunity. In mouse, there are eight known TIM genes (# 1-8) vs. only three genes in human (# 1, 3 & 4) (1, 2). Mouse TIM‑1 and ‑2 are counterparts of human TIM‑1, while mouse TIM‑5 through TIM‑8 have no human counterparts (2). Mouse TIM‑1 (isoform 2) is synthesized as a 282 amino acid (aa) precursor that contains a 21 aa signal sequence, a 193 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 47 aa cytoplasmic domain (5, 6). The ECD contains one V‑type Ig‑like domain and a mucin region characterized by multiple T‑S‑P motifs. The mucin region undergoes extensive O‑linked glycosylation. The mouse TIM‑1 gene is highly polymorphic and, based on rat, may undergo alternate splicing (4, 6). One isoform (termed isoform 1) possesses a 23 aa insertion after Pro182 (GenBank # NP_599099). Another splice variant (of isoform 1) shows a 15 aa deletion in the mucin region of the ECD (6). This difference is associated with a decreased susceptibility to asthma. In human, TIM‑1 is known to circulate as a soluble form that arises from cleavage by an undefined MMP, releasing an 85 ‑ 90 kDa soluble molecule (7). In mouse, a 60-65 kDa soluble form has also been detected (in urine) that presumably arises from proteolytic processing (8). In‑house data from R&D Systems Inc. has demonstrated the presence of soluble TIM‑1 in mouse circulation. The ECD of mouse TIM‑1 shares 37% and 81% aa sequence identity with human and rat TIM‑1 ECD, respectively. Reported ligands for TIM‑1 include TIM‑4, phosphatidylserine, P-Selectin and the hepatitis A virus (3, 9, 10, 11). TIM‑1 ligation induces T cell proliferation and promotes cytokine production (1, 12). In particular, it induces IL‑4 production, and requires the TIM‑1 cytoplasmic tyrosine phosphorylation motif (5). TIM-1 also serves as a cellular entry receptor for various viruses, including hepatitis A virus, Ebolavirus, Marburgvirus and has been indicated as a possible receptor for SARS-CoV-2 (9, 13, 14).

References

  1. Meyers, J.H. et al. (2005) Trends Mol. Med. 11:1471.
  2. Su, E.W. et al. (2008) Cytokine 44:9.
  3. Freeman, G.J. et al. (2010) Immunol. Rev. 235:172.
  4. Ichimura, T. et al. (1998) J. Biol. Chem. 273:4135.
  5. de Souza, A.J. et al. (2005) Proc. Natl. Acad. Sci. USA 102:17113.
  6. McIntire, J.J. et al. (2001) Nat. Immunol. 2:1109.
  7. Bailly, V. et al. (2002) J. Biol. Chem. 277:39739.
  8. Herzog, C. et al. (2007) Kidney Int. 71:1009.
  9. Feigelstock, D. et al. (1998) J. Virol. 72:6621.
  10. Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
  11. Angiari, S. et al. (2014) J. Immuni. 40:542
  12. Meyers, J.H. et al. (2005) Nat. Immunol. 6:455.
  13. Kondratowicz, A.S. et al. (2011) Proc. Natl. Acad. Sci. USA 108:8426.
  14. Ichimura, T. et al. (2020) doi: 10.1101/2020.09.16.20190694. Preprint.

Long Name

T Cell Immunoglobulin Mucin-1

Alternate Names

CD365, HAVCR1, KIM-1, TIM1

Entrez Gene IDs

26762 (Human); 171283 (Mouse); 286934 (Rat); 102141332 (Cynomolgus Monkey)

Gene Symbol

HAVCR1

UniProt

NP_599009.2

Additional TIM-1/KIM-1/HAVCR Products

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Product Specific Notices for Recombinant Mouse TIM-1/KIM-1/HAVCR Isoform A Fc Protein, CF

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