Recombinant Mouse TIM-3 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 1529-TM

R&D Systems, part of Bio-Techne
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1529-TM-050
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Key Product Details

Source

NS0

Accession #

AAL65156

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Binding Activity

View all TIM-3 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse TIM-3 protein
Mouse TIM-3
(Leu22-Arg191)
Accession # AAL65156		 PIEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu22 & Asp24

Predicted Molecular Mass

45.6 kDa (monomer)

SDS-PAGE

67-80 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Immobilized Recombinant Human Galectin‑9 (Catalog # 2045-GA) at 500 ng/mL can bind recombinant mouse TIM-3 Fc Chimera with an apparent KD <10 nM.

Formulation, Preparation and Storage

1529-TM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIM-3

TIM-3 (T cell immunoglobulin and mucin domain-3), also known as HAVCR2, is a 60 kDa member of the TIM family of immune regulating molecules. TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk region (1, 2). Mature mouse TIM-3 consists of a 174 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 67 aa cytoplasmic tail (3). Within the ECD, mouse TIM-3 shares 58% and 75% aa sequence identity with human and rat TIM-3, respectively. TIM-3 is up‑regulated on several populations of activated myeloid cells (macrophage, monocyte, dendritic cell, microglia, mast cell) and T cells (Th1, CD8+, NK, Treg) (4‑11). Its binding to Galectin-9 induces a range of immunosuppressive functions which enhance immune tolerance and inhibit anti-tumor immunity (12). TIM-3 ligation attenuates CD8+ and Th1 cell responses (12-14) and promotes the activity of Treg and myeloid derived suppressor cells (9, 12, 14, 15). In addition, dendritic cell-expressed TIM-3 dampens inflammation by enabling the phagocytosis of apoptotic cells and the cross‑presentation of apoptotic cell antigens (4). It also binds the alarmin HMGB1, thereby preventing the activation of TLRs in response to released tumor cell DNA (5). TIM-3 interactions with Galectin-9 can alternatively trigger immune stimulatory effects, such as the coactivation of NK cell cytotoxicity (11).

References

  1. Sakuishi, K. et al. (2011) Trends Immunol. 32:345.
  2. Anderson, A.C. (2012) Curr. Opin. Immunol. 24:213.
  3. McIntire, J.J. et al. (2001) Nat. Immunol. 2:1109.
  4. Nakayama, M. et al. (2009) Blood 113:3821.
  5. Anderson, A.C. et al. (2007) Science 318:1141.
  6. Wiener, Z. et al. (2007) J. Invest. Dermatol. 127:906.
  7. Chiba, S. et al. (2012) Nat. Immunol. 13:832.
  8. Monney, L. et al. (2002) Nature 415:536.
  9. Sanchez-Fueyo, A. et al. (2003) Nat. Immunol. 4:1093.
  10. Ndhlovu, L.C. et al. (2012) Blood 119:3734.
  11. Gleason, M.K. et al. (2012) Blood 119:3064.
  12. Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
  13. Sakhdari, A. et al. (2012) PLoS ONE 7:e40146.
  14. Sabatos, C.A. et al. (2003) Nat. Immunol. 4:1102.
  15. Dardalhon, V. et al. (2010) J. Immunol. 185:1383.

Long Name

T Cell Immunoglobulin Mucin-3

Alternate Names

CD366, HAVcr-2, HAVCR2, KIM-3, SPTCL, TIM3, TIMD3

Entrez Gene IDs

84868 (Human); 171285 (Mouse); 363578 (Rat); 479318 (Canine); 102141722 (Cynomolgus Monkey)

Gene Symbol

HAVCR2

UniProt

AAL65156

Additional TIM-3 Products

Product Documents for Recombinant Mouse TIM-3 Fc Chimera Protein, CF

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Certificate of Analysis

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Product Specific Notices for Recombinant Mouse TIM-3 Fc Chimera Protein, CF

For research use only

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