Recombinant Rabbit IL-1 beta/IL-1F2 Protein

R&D Systems, part of Bio-Techne | Catalog # 7464-RB

R&D Systems, part of Bio-Techne
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Carrier Free
7464-RB-010/CF
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With Carrier
7464-RB-010
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Key Product Details

Source

E. coli

Accession #

NP_001075670

Conjugate

Unconjugated

Applications

Bioactivity

View all IL-1 beta/IL-1F2 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived rabbit IL-1 beta/IL-1F2 protein
Ala117-Ser268

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.1 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala117

Predicted Molecular Mass

17.5 kDa

SDS-PAGE

19 kDa, reducing conditions

Activity

Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272.
The ED50 for this effect is 3-18 pg/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 7464-RB
Formulation Lyophilized from a 0.2 μm filtered solution in MES, NaCl, DTT and EDTA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile, deionized water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 7464-RB/CF
Formulation Lyophilized from a 0.2 μm filtered solution in MES, NaCl, DTT and EDTA.
Reconstitution Reconstitute at 100 μg/mL in sterile, deionized water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1 beta/IL-1F2

IL‑1 is a name that designates two pleiotropic cytokines, IL‑1 alpha (IL‑1F1) and IL‑1 beta (IL‑1F2), which are the products of distinct genes. IL‑1 alpha and IL‑1 beta are structurally related polypeptides that share approximately 25% amino acid (aa) identity in rabbit. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL‑1 alpha and IL‑1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL‑1 RI binds directly to IL‑1 alpha or IL‑1 beta and then associates with IL‑1 R accessory protein (IL‑1 R3/IL‑1 RAcP) to form a high-affinity receptor complex that is competent for signal transduction. IL‑1 RII has high affinity for IL‑1 beta but functions as a decoy receptor and negative regulator of IL‑1 beta activity. IL‑1ra functions as a competitive antagonist by preventing IL‑1 alpha and IL‑1 beta from interacting with IL‑1 RI (1‑3). The rabbit IL‑1 beta cDNA encodes a 268 aa precursor. A 116 aa propeptide is cleaved intracellularly by the cysteine protease IL‑1 beta‑converting enzyme (Caspase‑1/ICE) to generate the active cytokine (4, 5). The 17 kDa mature rabbit IL‑1 beta shares 78%‑81% aa sequence identity with human, mouse, and rat and 64%‑72% with bovine, equine, feline, goat, human, ovine, and porcine IL‑1 beta.

References

  1. Dinarello, C.A. (2009) Annu. Rev. Immunol. 27:519.
  2. Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
  3. Weber, A. et al. (2010) Sci. Signal. 3:cm1.
  4. Cannon, J.G. et al. (1989) J. Immunol. 142:2299.
  5. Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.

Long Name

Interleukin 1 beta

Alternate Names

IL-1b, IL-1F2, IL1 beta, IL1B

Entrez Gene IDs

3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)

Gene Symbol

IL1B

UniProt

NP_001075670

Additional IL-1 beta/IL-1F2 Products

Product Documents for Recombinant Rabbit IL-1 beta/IL-1F2 Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 7464-RB (with carrier)
Download SDS 7464-RB/CF (carrier free)

Product Specific Notices for Recombinant Rabbit IL-1 beta/IL-1F2 Protein

For research use only

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